Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Science, Nanjing Normal University, Nanjing, 210046, People's Republic of China.
World J Microbiol Biotechnol. 2012 Feb;28(2):575-84. doi: 10.1007/s11274-011-0850-z. Epub 2011 Jul 30.
The effects of the endophytic fungus Gilmaniella sp. and its elicitor on the defense and metabolic responses of host plants Atractylodes lancea were investigated, in order to understand how to utilize endophytic fungi and their elicitor resources better. The results showed that the promotion effect of the fungus on the growth of host plantlets was much better than that of its elicitor. Both fungus and elicitor enhanced defense-related enzyme activities. In fungus-inoculated groups, phenylalanine ammonia lyase and polyphenol oxidase activities increased slowly, and reached a maximum level during the later stages, whereas peroxidase activity peaked in the first few days. Additionally, the activities of chitinase and β-1,3-glucanase were significantly higher than those of the control plants. In elicitor-treated groups, however, most of the enzymes were activated during the early stage, and their highest levels were generally lower than those of the fungus-inoculated groups. Compared with the elicitor, fungal infection improved the photosynthetic rate of the host, and increased carbohydrate levels as well as chlorophyll content in host leaves. The total content of the four main components of volatile oil was also increased in elicitor-treated groups, but there was no particular pattern in this increase. Meanwhile, in the fungus-inoculated groups, the content of atractylone significantly increased with time, while the content of β-eudesmol decreased. These results indicated that fungal elicitor could substantially improve the total content of volatile oil, while the fungus could more effectively enhance the quality of herbal medicines.
研究了内生真菌 Gilmaniella sp. 及其诱导子对宿主植物白术防御和代谢反应的影响，以期更好地利用内生真菌及其诱导子资源。结果表明，真菌对宿主苗生长的促进作用明显优于其诱导子。真菌和诱导子均增强了防御相关酶的活性。在接种真菌的组中，苯丙氨酸解氨酶和多酚氧化酶活性缓慢增加，在后期达到最高水平，而过氧化物酶活性在最初几天达到峰值。此外，几丁质酶和β-1,3-葡聚糖酶的活性明显高于对照植物。然而，在诱导子处理组中，大多数酶在早期被激活，其最高水平通常低于接种真菌的组。与诱导子相比，真菌感染提高了宿主的光合作用，增加了宿主叶片中的碳水化合物水平和叶绿素含量。在诱导子处理组中，挥发油的四种主要成分的总含量也增加了，但增加没有特定的模式。同时，在接种真菌的组中，苍术酮的含量随时间显著增加，而β-桉叶醇的含量减少。这些结果表明，真菌诱导子可以显著提高挥发油的总含量，而真菌可以更有效地提高中药材的质量。
