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基于SRAP的不同居群温郁金遗传多样性研究

[Study on genetic diversity of Curcuma wenyujin from various habitats by SRAP].

作者信息

Leng Chun-Hong, Tao Zheng-Ming, Wu Zhi-Gang, Lin Xin-Chun, Lou Yong-Feng, Jiang Cheng-Xi

机构信息

Zhejiang Institution of Subtropical Crops, Wenzhou 325005, China.

出版信息

Zhong Yao Cai. 2009 Oct;32(10):1507-10.

PMID:20112709
Abstract

OBJECTIVE

The DNA fingerprints of Curcuma wenyujin from various habitats were generated by using SRAP markers to find the feasibility in analyzing their relationship.

METHODS

The DNA polymorphism of Curcuma wenyujin from various habitats were detected by SRAP molecular markers.

RESULTS

We had screened five pairs of primer combinations, and built the DNA fingerprints of Curcuma wenyujin from various habitats. The phylogenetic clustering results revealed that the genetic difference in Curcuma wenyujin from various habitats were little.

CONCLUSION

SRAP markers may offer some evidence for protecting and exploiting of Curcuma wenyuji.

摘要

目的

利用SRAP标记构建不同产地温郁金的DNA指纹图谱,探索分析其亲缘关系的可行性。

方法

采用SRAP分子标记技术检测不同产地温郁金的DNA多态性。

结果

筛选出5对引物组合,构建了不同产地温郁金的DNA指纹图谱。系统聚类分析结果表明,不同产地温郁金的遗传差异较小。

结论

SRAP标记可为温郁金的保护与开发利用提供一定依据。

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