Tham L G, Perumal N, Syed M A, Shamaan N A, Shukor M Y
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):135-8.
An inhibitive assay of insecticides using Acetylcholinesterase (AChE) from the local fish Clarias batrachus is reported. AChE was assayed according to the modified method of Ellman. Screening of insecticide and heavy metals showed that carbofuran and carbaryl strongly inhibited C. batrachus AChE. The inhibition concentration (IC) IC50 values (and the 95% confidence interval) for both carbofuran and carbaryl inhibition on C. batrachus AChE at 6.66 (5.97-7.52) and 130.00 (119.3-142.5) microg l(-1), respectively was within the IC50 range of Electrophorus electricus at 6.20 (6.03-6.39) and 133.01 (122.40-145.50) microg l(-1), respectively and were much lower than bovine AChE at 20.94 (19.53-22.58) and 418.80 (390.60-451.60) microg l(-1), respectively. The results showed that C. batrachus have the potential to be used as a cheaper and more readily available source of AChE than other more commercially available sources.
报道了一种使用当地鱼类胡子鲶的乙酰胆碱酯酶（AChE）对杀虫剂进行抑制性测定的方法。按照Ellman的改良方法测定AChE。对杀虫剂和重金属的筛选表明，克百威和西维因能强烈抑制胡子鲶的AChE。克百威和西维因对胡子鲶AChE的抑制浓度（IC）IC50值（及95%置信区间）分别为6.66（5.97 - 7.52）和130.00（119.3 - 142.5）μg l(-1)，分别在电鳗的IC50范围内，电鳗的IC50值分别为6.20（6.03 - 6.39）和133.01（122.40 - 145.50）μg l(-1)，且远低于牛AChE的IC50值，牛AChE的IC50值分别为20.94（19.53 - 22.58）和418.80（390.60 - 451.60）μg l(-1)。结果表明，与其他更商业化的来源相比，胡子鲶有潜力作为一种更便宜且更容易获得的AChE来源。
