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爪哇无须魮肝脏胆碱酯酶用于金属离子检测的评估

The assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ions.

作者信息

Sabullah Mohd Khalizan, Sulaiman Mohd Rosni, Abd Shukor Mohd Yunus, Syed Mohd Arif, Shamaan Nor Aripin, Khalid Ariff, Ahmad Siti Aqlima

机构信息

Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia ; Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.

Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.

出版信息

ScientificWorldJournal. 2014;2014:571094. doi: 10.1155/2014/571094. Epub 2014 Oct 27.

DOI:10.1155/2014/571094
PMID:25401148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4225846/
Abstract

Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and -25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions.

摘要

使用普鲁卡因酰胺 - 琼脂糖6B对爪哇魮肝脏中的胆碱酯酶粗提物进行纯化。选择S - 丁酰硫代胆碱碘化物（BTC）作为该测定的特异性合成底物，其最大速度最高，双分子常数最低，分别为53.49微摩尔/分钟/毫克和0.23毫摩尔，催化效率比为0.23。在pH 7.5和25至30°C范围内的最佳温度下获得最佳参数。评估了不同储存条件的影响，在室温下储存9天后胆碱酯酶活性显著降低。然而，在4.0、0和 - 25°C下储存15天，胆碱酯酶活性没有显著差异。重金属筛选表明，铬、铜和汞通过将活性降低至50%以下强烈抑制爪哇魮胆碱酯酶，而几种金属离子的两两组合对该酶表现出协同抑制作用，其作用大于单次暴露，尤其是铬、铜和汞。结果表明，爪哇魮胆碱酯酶有潜力用作检测金属离子的生物传感器。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7370477c96da/TSWJ2014-571094.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/bda50b3293cd/TSWJ2014-571094.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7048e1689cbe/TSWJ2014-571094.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7ae303b00be3/TSWJ2014-571094.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/b5efa6bc8ba8/TSWJ2014-571094.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7904f25b0229/TSWJ2014-571094.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/ba740b1c20c1/TSWJ2014-571094.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7370477c96da/TSWJ2014-571094.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/bda50b3293cd/TSWJ2014-571094.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7048e1689cbe/TSWJ2014-571094.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7ae303b00be3/TSWJ2014-571094.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/b5efa6bc8ba8/TSWJ2014-571094.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7904f25b0229/TSWJ2014-571094.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/ba740b1c20c1/TSWJ2014-571094.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e27/4225846/7370477c96da/TSWJ2014-571094.007.jpg

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