Onbasli Dilsad, Aslim Belma
Deparment of Biology, Faculty of Science and Arts, Kastamonu University, Kastamonu, Turkey.
J Environ Biol. 2009 Jan;30(1):161-3.
In this study rhamnolipid biosurfactant production was investigated in eighteen strains of Pseudomonas spp.. Rhamnolipid by these strains was determined by a spectrophotometric method in nutrient broth medium (NB). From the 18 strains screened, two Pseudomonas strains (Pseudomonas luteola B17 and Pseudomonas putida B12) which had produced the highest percentage yield of rhamnolipid were examined for rhamnolipid production at different incubation times (24, 48 and 72 hr) and different sugar beet molasses concentrations [1-5% w/v concentration (1-5 g molasses/100 ml water)]. The rhamnolipid production increased with the increase in the concentration of molasses and maximum production occurred when 5 % (w/v) of molasses were used. At the same time, maximum rhamnolipid production occurred after 72 hr of incubation. When the amount of rhamnolipid produced at different incubation times (24, 48 and 72 hr) and with different concentrations of molasses [1-5 % w/v concentration (1-5 g molasses/100 ml water)] by Pseudomonas spp.; was compared, no significant difference in amount of production was seen. These studies show that the waste product from sugar industry may be suggested for important biotechnological processes such as rhamnolipid production.
在本研究中，对18株假单胞菌属菌株的鼠李糖脂生物表面活性剂产量进行了研究。这些菌株产生的鼠李糖脂通过分光光度法在营养肉汤培养基（NB）中测定。从筛选出的18株菌株中，选取了两株产生鼠李糖脂百分比产量最高的假单胞菌菌株（浅黄金色假单胞菌B17和恶臭假单胞菌B12），检测它们在不同培养时间（24、48和72小时）以及不同甜菜糖蜜浓度[1 - 5%（w/v）浓度（1 - 5克糖蜜/100毫升水）]下的鼠李糖脂产量。鼠李糖脂产量随糖蜜浓度的增加而增加，当使用5%（w/v）的糖蜜时产量最高。同时，在培养72小时后鼠李糖脂产量最高。比较假单胞菌属在不同培养时间（24、48和72小时）以及不同糖蜜浓度[1 - 5%（w/v）浓度（1 - 5克糖蜜/100毫升水）]下产生的鼠李糖脂量，产量上没有显著差异。这些研究表明，制糖工业的废料可用于鼠李糖脂生产等重要生物技术过程。
