Department of Environmental Protection, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences-SGGW, Ul. Nowoursynowska 159, 02-776 Warsaw, Poland.
Int J Food Sci Nutr. 2010 Jun;61(4):381-94. doi: 10.3109/09637480903517788.
The present research aimed at evaluating the vitamin C, total phenolic content (TPC), phenolic compounds, carotenoids, and chlorophyll contents, as well as antioxidant activity (AAC) of six Actinidia species fruit. Vitamin C, phenolic compounds, carotenoids and chlorophylls were measured using high-performance liquid chromatography. TPC was determined using the Folin-Ciocalteau reagent, and AAC using 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay. The highest concentrations of vitamin C and TPC were found for Actinidia kolomikta fruit (1008.3 and 634.1 mg/100 g fresh weight [FW], respectively). Among phenolic compounds, seven phenolic acids and three flavonoids were identified. The 2,5-dihydroxybenzoic acid prevailed in A. kolomikta (425.54 mg/100 g FW), while tannic acid dominated in other species (4.63-100.43 mg/100 g FW). The largest amounts of chlorophylls and carotenoids were identified as Actinidia macrosperma (4.02 and 2.09 mg/100 g FW, respectively). The AAC of fruit extracts decreased in the order of A. kolomikta > Actinidia purpurea > Actinidia melanandra > A. macrosperma > Actinidia arguta > Actinidia deliciosa according to the DPPH assay.
本研究旨在评估 6 种猕猴桃属水果的维生素 C、总酚含量（TPC）、酚类化合物、类胡萝卜素和叶绿素含量以及抗氧化活性（AAC）。采用高效液相色谱法测定维生素 C、酚类化合物、类胡萝卜素和叶绿素。采用福林-考尔莱特试剂测定 TPC，采用 2,2-二苯基-1-苦基肼（DPPH）法测定 AAC。维生素 C 和 TPC 的含量最高的是软枣猕猴桃（1008.3 和 634.1 mg/100 g 鲜重[FW]）。在酚类化合物中，鉴定出 7 种酚酸和 3 种类黄酮。2,5-二羟基苯甲酸在软枣猕猴桃中含量最高（425.54 mg/100 g FW），而鞣酸在其他种中含量最高（4.63-100.43 mg/100 g FW）。叶绿素和类胡萝卜素含量最高的是狗枣猕猴桃（分别为 4.02 和 2.09 mg/100 g FW）。根据 DPPH 试验，水果提取物的 AAC 顺序为软枣猕猴桃>中华猕猴桃>深山猕猴桃>狗枣猕猴桃>毛花猕猴桃>美味猕猴桃。
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