Department of Environment Protection, Faculty of Horticulture and Landscape Architecture, Warsaw University of Life Sciences, Warsaw, Poland.
J Sci Food Agric. 2013 Apr;93(6):1412-9. doi: 10.1002/jsfa.5909. Epub 2012 Oct 15.
Hardy kiwifruit (Actinidia arguta) is a new species, commercially grown in recent years. Total phenolics (TPC), vitamin C (TAA) content, antioxidant activity (AA) and their year-to-year variability in seven hardy kiwifruit clones were evaluated. TPC was determined using the Folin-Ciocalteu reagent assay. TAA was estimated by determination of l-ascorbic acid and l-dehydroascorbic acid levels using high-performance liquid chromatography. AA was measured using diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and OH radicals.
The highest content of vitamin C, in all seasons, was found in D11 (1447-2181 mg kg(-1) fresh weight) and phenolics for D11 and M1 clones (2583-3312 and 2228-3414 mg gallic acid equivalents kg(-1) fresh weight, respectively). TPC and TAA content showed significant differences between hardy kiwifruit clones and showed significant year-to-year variability. Each year, the level of AA was significantly higher for D11 (DPPH, ABTS). AA was strongly correlated with TPC and TAA content in Actinidia fruit.
Hardy kiwifruit are an important source of vitamin C and phenolics, which resulted in their good antioxidant potential. A significantly higher content of these compounds was found in fruit of hybrid origin, which suggests that A. purpurea × A. arguta clones may be useful genetic resources for further interspecific hybridization.
海沃德猕猴桃（Actinidia arguta）是近年来商业种植的一个新物种。本研究评估了 7 个海沃德猕猴桃无性系的总酚（TPC）、维生素 C（TAA）含量、抗氧化活性（AA）及其年度变异性。采用福林-肖卡试剂法测定 TPC，高效液相色谱法测定 l-抗坏血酸和 l-脱氢抗坏血酸水平来估算 TAA，采用二苯基-1-苦基肼（DPPH）、2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和 OH 自由基法测定 AA。
在所有季节中，D11 猕猴桃的维生素 C 含量最高（1447-2181mgkg(-1)鲜重），D11 和 M1 猕猴桃的酚类物质含量最高（2583-3312 和 2228-3414mg 没食子酸当量 kg(-1)鲜重）。TPC 和 TAA 含量在不同猕猴桃无性系间存在显著差异，且具有显著的年度变异性。每年，D11 猕猴桃的 AA 水平（DPPH、ABTS）均显著较高。AA 与猕猴桃果实中的 TPC 和 TAA 含量呈显著正相关。
海沃德猕猴桃是维生素 C 和酚类物质的重要来源，这使其具有良好的抗氧化潜力。杂交起源的果实中这些化合物的含量明显较高，这表明 A. purpurea×A. arguta 无性系可能是进一步种间杂交的有用遗传资源。
