用于活细胞中过氧亚硝酸盐检测与成像的基于硼二吡咯亚甲基的荧光探针。
BODIPY-based fluorescent probe for peroxynitrite detection and imaging in living cells.
作者信息
Sun Zhen-Ning, Wang Hua-Li, Liu Feng-Qin, Chen Yan, Tam Paul Kwong Hang, Yang Dan
机构信息
Morningside Laboratory for Chemical Biology, Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, People's Republic of China.
出版信息
Org Lett. 2009 May 7;11(9):1887-90. doi: 10.1021/ol900279z.
A fluorescent probe, HKGreen-2, has been developed based on a specific reaction between ketone and peroxynitrite (ONOO(-)). This probe is highly sensitive and selective for the detection of peroxynitrite not only in abiotic but also in biological systems. With this probe, we successfully detected peroxynitrite generated in murine macrophage cells activated by phorbol 12-myristate 13-acetate (PMA), interferon-gamma (IFN-gamma), and lipopolysaccharide (LPS). This new probe will be a useful tool for studying the roles of peroxynitrite in biological processes.
一种荧光探针HKGreen-2已基于酮与过氧亚硝酸盐（ONOO(-)）之间的特定反应开发出来。该探针不仅对非生物体系，而且对生物体系中过氧亚硝酸盐的检测都具有高度的敏感性和选择性。利用该探针，我们成功检测到了由佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）、干扰素-γ（IFN-γ）和脂多糖（LPS）激活的小鼠巨噬细胞中产生的过氧亚硝酸盐。这种新探针将成为研究过氧亚硝酸盐在生物过程中作用的有用工具。
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