Li Guanglei, Li Jianye, Otsuka Yu, Zhang Shuai, Takahashi Masashi, Yamada Koji
Division of Materials Science, Graduate School of Environmental Science, Hokkaido University, Sapporo 0860-0810, Japan.
Laboratory of Animal Genetics and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Materials (Basel). 2020 Feb 3;13(3):677. doi: 10.3390/ma13030677.
We developed an easily accessible boron-dipyrromethene (BODIPY)-based fluorogenic probe, which we named LD-TB. This probe emits bright fluorescence in oil; when compared with aqueous solution, a significant enhancement of fluorescence brightness is observed. Cellular experiments confirmed that the probe stains the lipid droplets (LDs) specifically in both live and fixed cells, providing background-free images. Compared with Nile Red dye, a commonly used LD marker, LD-TB showed superior photostability. The sharp absorption and emission bands enable its multicolor imaging with blue and green probes. Importantly, the probe has proved to have low toxicity and is compatible with cell fixation. Our research provides a promising new fluorogenic probe for specific imaging of LDs.
我们开发了一种易于获取的基于硼二吡咯亚甲基（BODIPY）的荧光探针，我们将其命名为LD-TB。该探针在油中发出明亮的荧光；与水溶液相比，观察到荧光亮度有显著增强。细胞实验证实，该探针在活细胞和固定细胞中均能特异性地标记脂滴（LDs），提供无背景图像。与常用的LD标记尼罗红染料相比，LD-TB表现出优异的光稳定性。其尖锐的吸收和发射带使其能够与蓝色和绿色探针进行多色成像。重要的是，该探针已被证明具有低毒性，并且与细胞固定兼容。我们的研究为LDs的特异性成像提供了一种有前景的新型荧光探针。
