School of Chemistry and Chemical Engineering, Henan University of Technology, Lianhua Road, Zhengzhou, 450001, China.
Anal Chim Acta. 2010 Feb 28;661(2):215-21. doi: 10.1016/j.aca.2009.12.029. Epub 2009 Dec 29.
An analysis method is reported for dibutyl phthalate and related compounds with high selectivity and sensitivity by using the selective molecularly imprinted solid-phase extraction (MISPE) technique. In this report, dibutyl phthalate (DBP) is employed as the template molecule, and the molecularly imprinted polymers (MIPs) are synthesized through the bulk polymerization of methacrylic acid (MAA). The Scatchard plot suggests that the template-polymer system has two-site binding behavior with the dissociation constants of 0.5187 and 0.01898 mmol L(-1), respectively. The rebinding test, based on the MISPE column technique, shows the recoveries of soybean milk samples spiked with 5 phthalates are in the range of 75.8-107.5% with the relative standard deviations of 1.80-10.08%, indicating the feasibility of the prepared MIPs for phthalates extraction. Finally, the method is used to analyze the trace level of phthalates in commercial soybean milk.
本文报道了一种高选择性和高灵敏度的邻苯二甲酸二丁酯及相关化合物的分析方法，该方法采用选择性分子印迹固相萃取（MISPE）技术。在本报告中，邻苯二甲酸二丁酯（DBP）被用作模板分子，通过甲基丙烯酸（MAA）的本体聚合合成了分子印迹聚合物（MIPs）。Scatchard 图表明，模板-聚合物体系具有双位点结合行为，解离常数分别为 0.5187 和 0.01898 mmol·L（-1）。基于 MISPE 柱技术的再结合试验表明，用 5 种邻苯二甲酸对豆奶样品进行加标回收，回收率在 75.8%-107.5%之间，相对标准偏差为 1.80%-10.08%，表明所制备的 MIPs 可用于邻苯二甲酸酯的提取。最后，该方法用于分析商业豆奶中痕量水平的邻苯二甲酸酯。
