Jhu Shih-Ci, Wang Jing-Ying, Wang Hsueh-Ting, Chen Sung-Fang
Department of Chemistry, National Taiwan Normal University, Taipei, 11677, Taiwan.
Instrumentation Center, National Taiwan Normal University, Taipei, 11677, Taiwan.
J Food Drug Anal. 2021 Dec 15;29(4):726-737. doi: 10.38212/2224-6614.3384.
Heterocyclic aromatic amines (HCAs) are highly carcinogenic and mutagenic chemicals. This study reports on the development of magnetic molecularly imprinted polymers (MMIPs) for the purification and quantification of HCAs. A novel magnetic molecularly imprinted polymer was successfully prepared using a surface molecular imprinting method using functionalized Fe particles as the magnetic cores. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) was used as a molecular template; methacrylic acid (MAA), ethylene glycol dimethyl acrylate (EGDMA), 2, 2'-Azobis (2-methylpropionitrile) were used as the functional monomer, crosslinker, and initiator, respectively. The use of the template/functional monomer/crosslinking agent at a ratio of 1:4:20 resulted in a product with better adsorption properties (3.24 mg/g). The HCAs were successfully detected and quantified in processed meat samples by MISPE and LC-MS/MS. Under the final optimized detection conditions, the proposed method offered good linearity (R > 0.995) for the five HCAs with an acceptable level of precision, and an LOQ of 0.05 ng/g was successfully achieved.
杂环芳香胺（HCAs）是具有高度致癌性和致突变性的化学物质。本研究报道了用于HCAs纯化和定量的磁性分子印迹聚合物（MMIPs）的开发。采用表面分子印迹法，以功能化铁颗粒为磁核，成功制备了一种新型磁性分子印迹聚合物。以2-氨基-3-甲基咪唑并[4,5-f]喹啉（IQ）为分子模板；分别以甲基丙烯酸（MAA）、乙二醇二甲基丙烯酸酯（EGDMA）、2,2'-偶氮二异丁腈为功能单体、交联剂和引发剂。模板/功能单体/交联剂以1:4:20的比例使用，得到了具有更好吸附性能（3.24 mg/g）的产物。通过MISPE和LC-MS/MS成功检测并定量了加工肉类样品中的HCAs。在最终优化的检测条件下，该方法对5种HCAs具有良好的线性（R>0.995），精密度可接受，成功实现了0.05 ng/g的定量限。
