Department of Medical Laboratory, Chongqing Medical University, Chongqing, PR China.
Exp Mol Pathol. 2010 Aug;89(1):58-62. doi: 10.1016/j.yexmp.2010.01.008. Epub 2010 Jan 28.
Prostate cancer (PCa) is the second most common cancer in men, and its incidence is still increasing. PCA3 is the most prostate cancer specific biomarker. Here we confirmed that both exon 3 and exon 4 are in the prostate-specific region of the PCA3 gene, and established the methodology of real-time fluorescent quantitative RT-PCR (FQ-RT-PCR) detecting PCA3 mRNA with primer spanning exons 1 and 3, and evaluated its clinical utility in a Chinese population. What disclosed that PCA3 mRNA is prostate cancer specific and shows increased expression in prostate cancer. It could be a reliable molecular marker in prostate cancer diagnosis. Exon 3-based real-time FQ-RT-PCR may prove useful in prostate cancer diagnosis, given that the associated primer would span only exons 1 and 3, relative to other models spanning exons 1 to 4. A shorter amplicon would not only enhance the efficiency of real-time FQ-RT-PCR, but may also simplify the quantification of PCA3 mRNA.
前列腺癌（PCa）是男性中第二常见的癌症，其发病率仍在上升。PCA3 是最具前列腺癌特异性的生物标志物。在这里，我们证实了外显子 3 和外显子 4 都位于 PCA3 基因的前列腺特异性区域，并建立了用跨越外显子 1 和 3 的引物实时荧光定量 RT-PCR（FQ-RT-PCR）检测 PCA3 mRNA 的方法，并在中国人中评估了其临床应用价值。结果表明 PCA3 mRNA 是前列腺癌特异性的，在前列腺癌中表达增加。它可能是前列腺癌诊断中可靠的分子标志物。基于外显子 3 的实时 FQ-RT-PCR 可能在前列腺癌诊断中有用，因为相关引物仅跨越外显子 1 和 3，而其他模型则跨越外显子 1 至 4。较短的扩增子不仅可以提高实时 FQ-RT-PCR 的效率，还可以简化 PCA3 mRNA 的定量。
