Whitsett J A, Hull W M, Luse S
Division of Pulmonary Biology, Children's Hospital Medical Center, Cinncinati, Ohio.
Pediatrics. 1991 Apr;87(4):505-10.
Serum from premature infants enrolled in either single-dose or multidose surfactant replacement studies with bovine lung-based exogenous surfactant (Survanta) were analyzed for antibodies reactive with mixtures of bovine surfactant proteins SP-B and SP-C. Sera from 404 premature infants enrolled in single-dose studies and 1024 premature infants enrolled in multidose studies were analyzed, representing a total of 987 samples and 2743 serum samples, respectively. The sera were obtained from treated and control infants at the time of treatment and 1 week, 4 weeks, and 6 months thereafter. Polyclonal antisera generated in rabbits against the small molecular weight proteins were uniformly reactive with the bovine surfactant test antigens; however, antibodies reacting with the surfactant proteins were never detected by immunoblot analysis with the infant sera. Antibodies against common human viral antigens were readily detected in infant serum samples. The horseradish-peroxidase conjugated second antibodies (antihuman immunoglobin G or antihuman immunoglobins A, G, and M) used in the studies were highly reactive with both immunoglobin G and immunoglobin M classes of human antibodies. Therefore there was failure to detect specific immunological responses to the bovine surfactant proteins present in Survanta after single or multiple doses of exogenous surfactant administered in the perinatal period.
对参与单剂量或多剂量表面活性剂替代研究的早产儿血清进行分析，这些研究使用的是基于牛肺的外源性表面活性剂（固尔苏），检测其中与牛表面活性剂蛋白SP-B和SP-C混合物发生反应的抗体。分析了参与单剂量研究的404名早产儿血清以及参与多剂量研究的1024名早产儿血清，分别代表总共987个样本和2743份血清样本。这些血清是在治疗时以及治疗后1周、4周和6个月从治疗组和对照组婴儿身上获取的。用针对小分子蛋白在兔体内产生的多克隆抗血清与牛表面活性剂测试抗原呈一致反应；然而，通过婴儿血清免疫印迹分析从未检测到与表面活性剂蛋白发生反应的抗体。在婴儿血清样本中很容易检测到针对常见人类病毒抗原的抗体。研究中使用的辣根过氧化物酶偶联二抗（抗人免疫球蛋白G或抗人免疫球蛋白A、G和M）与人类抗体的免疫球蛋白G和免疫球蛋白M类别都有高度反应。因此，在围产期给予单剂量或多剂量外源性表面活性剂后，未能检测到对固尔苏中存在的牛表面活性剂蛋白的特异性免疫反应。
