Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.
作者信息
Crowe J S, Cooper H J, Smith M A, Sims M J, Parker D, Gewert D
机构信息
Department of Cell Biology, Wellcome Research Laboratories, Bechenham, Kent, UK.
出版信息
Nucleic Acids Res. 1991 Jan 11;19(1):184. doi: 10.1093/nar/19.1.184.
Abstract
摘要
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本文引用的文献
1
PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products.PCR诱导（无连接酶）亚克隆：一种快速可靠的亚克隆聚合酶链反应（PCR）产物的方法。
Nucleic Acids Res. 1990 Apr 11;18(7):1920. doi: 10.1093/nar/18.7.1920.
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