Unité de Biochimie Physiologique, Université Catholique de Louvain, Place Croix du Sud 2-20, B-1348, Louvain-la-Neuve, Belgium.
Theor Appl Genet. 1995 Nov;91(6-7):876-84. doi: 10.1007/BF00223895.
We have cloned a 4.5-kb mtDNA fragment showing a high RFLP polymorphism between various Hevea genotypes. Subcloning and sequencing of a 1.4-kb segment of this clone allowed us to design PCR amplification primers to isolate homologous mtDNA segments of about 0.9 kb from 23 representative genotypes of Hevea. Complete sequences from 4 genotypes showed between 6.7% and 20.2% of nucleotide diversity, suggesting the presence of a hypervariable, or hotspot, region. A sequence of 345 nucleotides within this region was determined for the 23 genotypes. The phylogenetic relationships inferred from the sequence comparison are in general agreement with the results obtained from mtDNA RFLP analysis, indicating that this polymorphic mtDNA region is a useful molecular marker for phylogenetic analysis within Hevea.
我们已经克隆了一个 4.5kb 的 mtDNA 片段，该片段在各种 Hevea 基因型之间表现出高度的 RFLP 多态性。对该克隆的一个 1.4kb 片段的亚克隆和测序，使我们能够设计 PCR 扩增引物，从 23 个代表性的 Hevea 基因型中分离出大约 0.9kb 的同源 mtDNA 片段。来自 4 个基因型的完整序列显示出 6.7%至 20.2%的核苷酸多样性，表明存在一个高度可变的或热点区域。对这个区域的 345 个核苷酸的序列进行了 23 个基因型的测定。从序列比较中推断出的系统发育关系与从 mtDNA RFLP 分析中获得的结果基本一致，表明这个多态性 mtDNA 区域是 Hevea 内系统发育分析的一个有用的分子标记。
