Wimmershoff J, Polkinghorne A, Grest P, Schade B, Marchal T, Keller S M, Guscetti F
Institute of Veterinary Pathology, Vetsuisse-Faculty, University of Zurich, CH-8032 Zurich, Switzerland.
J Comp Pathol. 2010 May;142(4):311-22. doi: 10.1016/j.jcpa.2009.12.004. Epub 2010 Feb 8.
Survivin is a member of the family of proteins known as 'inhibitors of apoptosis proteins'. Survivin has a role in cellular decisions concerning division and survival and is frequently expressed in neoplastic cells. The aim of the present study was to investigate immunohistochemically the expression of survivin in normal canine tissues and in canine lymphoma. A representative range of fetal and adult normal tissues as well as biopsy samples from dogs with lymphoma were assembled in tissue arrays. The lymphomas were classified according to the revised Kiel and to the Revised European American Lymphoma-World Health Organization (REAL-WHO) schemes. Polyclonal and monoclonal antisera cross-reactive with canine survivin identified cytoplasmic expression of the molecule in a broad range of normal canine cells. The same reagents demonstrated cytoplasmic labelling of more than 5% of cells in all 83 lymphoma samples tested with polyclonal antiserum and in 67 of 82 (82%) of samples tested with monoclonal antiserum. Survivin was expressed by a wide range of canine lymphoma subtypes, but the expression of this molecule in normal canine tissues must be considered if novel therapies targeting survivin are applied to the management of canine lymphoma.
生存素是“凋亡抑制蛋白”家族的一员。生存素在细胞的分裂和存活决策中发挥作用，且在肿瘤细胞中常呈高表达。本研究旨在通过免疫组织化学方法，研究生存素在正常犬组织和犬淋巴瘤中的表达情况。选取了一系列具有代表性的胎儿及成年正常组织，以及来自患淋巴瘤犬的活检样本制作组织芯片。淋巴瘤依据修订后的基尔分类法以及修订后的欧美淋巴瘤-世界卫生组织（REAL-WHO）分类方案进行分类。与犬生存素发生交叉反应的多克隆和单克隆抗血清，在多种正常犬细胞中识别出该分子的细胞质表达。相同试剂显示，在用多克隆抗血清检测的所有83个淋巴瘤样本中，以及在用单克隆抗血清检测的82个样本中的67个（82%）样本中，超过5%的细胞出现细胞质标记。多种犬淋巴瘤亚型均表达生存素，但如果将针对生存素的新型疗法应用于犬淋巴瘤的治疗，必须考虑该分子在正常犬组织中的表达情况。
