Bongiovanni Laura, Colombi Isabella, Fortunato Carmine, Della Salda Leonardo
Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, University of Teramo, Italy.
Vet Dermatol. 2009 Oct;20(5-6):369-76. doi: 10.1111/j.1365-3164.2009.00822.x.
Survivin, a member of the inhibitor of apoptosis protein (IAP) family, is ubiquitously expressed during tissue development, undetectable in most normal tissues, but re-expressed in most cancers, including skin malignancies. Expression of survivin was evaluated retrospectively in 19 canine cutaneous squamous cell carcinomas (SCCs; one in situ; 16 well differentiated; one invasive, one lymph node metastasis) and 19 well differentiated SCCs from human beings. Seven specimens of normal canine skin were included. Immunohistochemical expression of full-length survivin was determined using a commercially available antibody. In addition, apoptotic rate [Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labelling index (TUNEL) index] and mitotic index (MI), counting mitoses in 10 high power fields (HPF), were determined. Scattered survivin positive nuclei were identified in the epidermal basal cell layer of normal canine skin. Nuclear survivin expression was identified in 18 of 19 human and in all canine SCCs, mainly along the base of the tumour cell population. Cytoplasmic survivin expression was rarely observed in human SCCs and in 84.2% of canine SCCs. The TUNEL index ranged from 0.1 to 2.6 in human beings and from 7.5 to 69.4 in dogs, while MIs ranged from 0 to 4 in human beings and dogs. No correlation was found between survivin expression and apoptotic or mitotic rates. Canine and human tumours showed similar nuclear survivin expression, indicating similar functions of the molecule. We demonstrated survivin expression in normal adult canine epidermis. Increased nuclear survivin expression in pre-neoplastic and neoplastic lesions demonstrates a possible association of survivin with development of SCCs in human beings and dogs.
生存素是凋亡抑制蛋白（IAP）家族的成员之一，在组织发育过程中普遍表达，在大多数正常组织中无法检测到，但在包括皮肤恶性肿瘤在内的大多数癌症中重新表达。对19例犬皮肤鳞状细胞癌（SCC；1例原位癌；16例高分化；1例浸润性癌，1例淋巴结转移）和19例来自人类的高分化SCC进行了生存素表达的回顾性评估。纳入了7例正常犬皮肤标本。使用市售抗体测定全长生存素的免疫组化表达。此外，还测定了凋亡率[末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记指数（TUNEL）指数]和有丝分裂指数（MI），在10个高倍视野（HPF）中计数有丝分裂。在正常犬皮肤的表皮基底细胞层中发现了散在的生存素阳性细胞核。在19例人类SCC中的18例以及所有犬SCC中均发现了细胞核生存素表达，主要沿着肿瘤细胞群的底部。在人类SCC和84.2%的犬SCC中很少观察到细胞质生存素表达。人类的TUNEL指数范围为0.1至2.6，犬的为7.5至69.4，而人类和犬的MI范围均为0至4。未发现生存素表达与凋亡率或有丝分裂率之间存在相关性。犬和人类肿瘤显示出相似的细胞核生存素表达，表明该分子具有相似的功能。我们证明了生存素在正常成年犬表皮中的表达。肿瘤前病变和肿瘤病变中细胞核生存素表达的增加表明生存素可能与人类和犬SCC的发生有关。
