Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
Biomaterials. 2010 Apr;31(11):3103-13. doi: 10.1016/j.biomaterials.2010.01.013. Epub 2010 Feb 8.
Polysaccharide hybrids consisting of hyaluronic acid (HA) grafted with a dextran-tyramine conjugate (Dex-TA) were synthesized and investigated as injectable biomimetic hydrogels for cartilage tissue engineering. The design of these hybrids (denoted as HA-g-Dex-TA) is based on the molecular structure of proteoglycans present in the extracellular matrix of native cartilage. Hydrogels of HA-g-Dex-TA were rapidly formed within 2 min via enzymatic crosslinking of the tyramine residues in the presence of horseradish peroxidase and hydrogen peroxide. The gelation time, equilibrium swelling and storage modulus could be adjusted by varying the degree of substitution of tyramine residues and polymer concentration. Bovine chondrocytes incorporated in the HA-g-Dex-TA hydrogels remained viable, as shown by the Live-dead assay. Moreover, enhanced chondrocyte proliferation and matrix production were observed in the HA-g-Dex-TA hydrogels compared to Dex-TA hydrogels. These results suggest that HA-g-Dex-TA hydrogels have a high potential as injectable scaffolds for cartilage tissue engineering.
由透明质酸（HA）接枝葡聚糖-酪胺（Dex-TA）组成的多糖杂化物被合成并作为用于软骨组织工程的可注射仿生水凝胶进行了研究。这些杂化物（表示为 HA-g-Dex-TA）的设计基于存在于天然软骨细胞外基质中的蛋白聚糖的分子结构。在辣根过氧化物酶和过氧化氢存在下，通过对酪胺残基进行酶交联，HA-g-Dex-TA 水凝胶在 2 分钟内迅速形成。通过改变酪胺残基的取代度和聚合物浓度可以调节凝胶时间、平衡溶胀度和储能模量。如 Live-dead 测定所示，包埋在 HA-g-Dex-TA 水凝胶中的牛软骨细胞保持存活。此外，与 Dex-TA 水凝胶相比，在 HA-g-Dex-TA 水凝胶中观察到增强的软骨细胞增殖和基质产生。这些结果表明，HA-g-Dex-TA 水凝胶作为软骨组织工程的可注射支架具有很大的潜力。
