Department of Polymer Chemistry and Biomaterials, MIRA Institute for Biomedical Technology and Technical Medicine, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
J Control Release. 2011 May 30;152(1):186-95. doi: 10.1016/j.jconrel.2011.01.031. Epub 2011 Feb 1.
In this study, injectable hydrogels were prepared by horseradish peroxidase-mediated co-crosslinking of dextran-tyramine (Dex-TA) and heparin-tyramine (Hep-TA) conjugates and used as scaffolds for cartilage tissue engineering. The swelling and mechanical properties of these hydrogels can be easily controlled by the Dex-TA/Hep-TA weight ratio. When chondrocytes were incorporated in these gels, cell viability and proliferation were highest for gels with a 50/50 weight ratio of Dex-TA/Hep-TA. Moreover, these hydrogels induced an enhanced production of chondroitin sulfate and a more abundant presence of collagen as compared to Dex-TA hydrogels. The results indicate that injectable Dex-TA/Hep-TA hydrogels are promising scaffolds for cartilage regeneration.
在这项研究中，通过辣根过氧化物酶介导的葡聚糖-酪胺（Dex-TA）和肝素-酪胺（Hep-TA）缀合物的共交联制备了可注射水凝胶，并将其用作软骨组织工程的支架。这些水凝胶的溶胀和机械性能可以通过 Dex-TA/Hep-TA 的重量比轻松控制。当软骨细胞被掺入这些凝胶中时，Dex-TA/Hep-TA 重量比为 50/50 的凝胶的细胞活力和增殖率最高。此外，与 Dex-TA 水凝胶相比，这些水凝胶诱导硫酸软骨素的产生增加，并且胶原蛋白的存在更加丰富。结果表明，可注射的 Dex-TA/Hep-TA 水凝胶是软骨再生的有前途的支架。
