Humboldt-Universität zu Berlin, Institute of Physics, Photobiophysics, Newtonstr. 15, 12489 Berlin, Germany.
J Photochem Photobiol B. 2010 Mar 8;98(3):173-9. doi: 10.1016/j.jphotobiol.2009.11.013. Epub 2009 Dec 21.
The kinetics of chemical singlet oxygen quencher consumption inside living cells during low dose illumination was revealed via time resolved singlet oxygen luminescence detection. Deviations of the measured data from the common theoretical model for (1)O(2) kinetics forced the authors to consider a one-dimensional diffusion model for description of the kinetics of singlet oxygen generated by membrane localized photosensitizers. Our observations reconcile seemingly contradictory reports presenting different values for the efficiency of singlet oxygen interaction with cells.
通过时间分辨的单线态氧发光检测，揭示了在低剂量光照下活细胞内化学单线态氧猝灭剂消耗的动力学。测量数据与（1）O2 动力学的常见理论模型的偏差迫使作者考虑用一维扩散模型来描述膜定位的光敏剂产生的单线态氧的动力学。我们的观察结果调和了看似矛盾的报告，这些报告提出了与细胞相互作用的单线态氧效率的不同值。
