Baier Jürgen, Fuss Thomas, Pöllmann Claudia, Wiesmann Christopher, Pindl Katrin, Engl Roland, Baumer Daniela, Maier Max, Landthaler Michael, Bäumler Wolfgang
Universität Regensburg, Dermatologie, 93042 Regensburg, Germany.
J Photochem Photobiol B. 2007 Jun 26;87(3):163-73. doi: 10.1016/j.jphotobiol.2007.02.006. Epub 2007 Mar 31.
After the generation by different photosensitizers, the direct detection of singlet oxygen is performed by measuring its luminescence at 1270 nm. Using an infrared sensitive photomultiplier, the complete rise and decay time of singlet oxygen luminescence is measured at different concentrations of a photosensitizer, quencher, or oxygen. This allows the extraction of important information about the photosensitized generation of singlet oxygen and its decay, in particular at different oxygen concentrations. Based on theoretical considerations all important relaxation rates and rate constants were determined for the triplet T(1) states of the photosensitizers and for singlet oxygen. In particular, depending on the oxygen or quencher concentration, the rise or the decay time of the luminescence signal exhibit different meanings regarding the lifetime of singlet oxygen or triplet T(1)-state. To compare with theory, singlet oxygen was generated by nine different photosensitizers dissolved in either H2O, D2O or EtOD. When using H2O as solvent, the decaying part of the luminescence signal is frequently not the lifetime of singlet oxygen, in particular at low oxygen concentration. Since cells show low oxygen concentrations, this must have an impact when looking at singlet oxygen detection in vitro or in vivo.
在由不同的光敏剂产生单线态氧之后，通过测量其在1270nm处的发光来直接检测单线态氧。使用红外敏感光电倍增管，在不同浓度的光敏剂、猝灭剂或氧气条件下测量单线态氧发光的完整上升和衰减时间。这使得能够提取关于单线态氧的光敏化产生及其衰减的重要信息，特别是在不同氧气浓度下。基于理论考虑，确定了光敏剂的三重态T(1)以及单线态氧的所有重要弛豫速率和速率常数。特别是，根据氧气或猝灭剂的浓度，发光信号的上升或衰减时间对于单线态氧或三重态T(1)态的寿命具有不同的意义。为了与理论进行比较，使用溶解在H2O、D2O或EtOD中的九种不同光敏剂产生单线态氧。当使用H2O作为溶剂时，发光信号的衰减部分通常不是单线态氧的寿命，特别是在低氧浓度下。由于细胞显示出低氧浓度，这在体外或体内观察单线态氧检测时必然会产生影响。
