College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China.
Vaccine. 2010 Mar 24;28(15):2775-81. doi: 10.1016/j.vaccine.2010.01.037. Epub 2010 Jan 29.
We sought to determine susceptibility to highly pathogenic avian influenza (HPAI) H5N1 virus and to explore immune protection of inactivated H5N1 vaccine in streptozotocin-induced type 1 diabetic mice. Susceptibility of diabetic mice to an H5N1 virus was evaluated by comparing the median lethal dose (LD(50)) and the lung virus titers with those of the healthy after the viral infection. To evaluate the influence of diabetes on vaccination, diabetic and healthy mice were immunized once with an inactivated H5N1 vaccine and then challenged with a lethal dose of H5N1 virus. The antibody responses, survival rates, lung virus titers and body weight changes were tested. Mice with type 1 diabetes had higher lung virus titers and lower survival rates than healthy mice after H5N1 virus infection. Inactivated H5N1 vaccine induced protective antibody in diabetic mice, but the antibody responses were postponed and weakened. In spite of this, diabetic mice could be protected against the lethal virus challenge by a single dose of immunization when the amount of the antigen increased. These results indicated that type 1 diabetic mice were more susceptible to H5N1 influenza virus infection than healthy mice, and can be effectively protected by inactivated H5N1 vaccine with increased antigen.
我们旨在确定对高致病性禽流感（HPAI）H5N1 病毒的易感性，并探索灭活 H5N1 疫苗对 1 型糖尿病小鼠的免疫保护作用。通过比较感染病毒后健康小鼠和糖尿病小鼠的半数致死剂量（LD（50））和肺部病毒滴度来评估糖尿病小鼠对 H5N1 病毒的易感性。为了评估糖尿病对疫苗接种的影响，将糖尿病和健康小鼠用灭活的 H5N1 疫苗免疫一次，然后用致死剂量的 H5N1 病毒进行攻毒。检测了抗体反应、存活率、肺部病毒滴度和体重变化。与健康小鼠相比，1 型糖尿病小鼠在感染 H5N1 病毒后肺部病毒滴度更高，存活率更低。灭活的 H5N1 疫苗在糖尿病小鼠中诱导了保护性抗体，但抗体反应被推迟和减弱。尽管如此，当抗原量增加时，一剂免疫接种仍可使糖尿病小鼠免受致命病毒攻击的保护。这些结果表明，1 型糖尿病小鼠比健康小鼠更容易感染 H5N1 流感病毒，并且可以通过增加抗原的灭活 H5N1 疫苗得到有效保护。
