Center for Reproductive Medicine, Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
Fertil Steril. 2010 Nov;94(6):2141-6. doi: 10.1016/j.fertnstert.2009.12.030. Epub 2010 Feb 1.
To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS).
Prospective study.
SETTING(S): Tertiary care hospital.
PATIENT(S): Infertile men (n=101).
INTERVENTION(S): Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while group II (n=44) included men with seminal ROS levels (≥250 relative light units/sec/×10(6) sperm).
MAIN OUTCOME MEASURE(S): Seminal ROS, TAC, sperm DNA fragmentation, ROS/TAC score were measured.
RESULT(S): Group II had a high incidence of sperm DNA fragmentation than group I. The odds ratio of 1.25 for elevated ROS levels corresponded to >10% greater DNA fragmentation in our patients (95% confidence interval 1.01-1.53). Group II showed poor motility, a higher incidence of leukocytospermia, and higher ROS-TAC scores compared with group I. ROS was negatively correlated with sperm curvilinear velocity (r=-.24), linearity (r=-.24), and sperm motility (r=-.31). Sperm motility was correlated with %TUNEL(+ve) sperm (r=-.39).
CONCLUSION(S): An increase in seminal ROS levels by 25% was associated with a 10% increase in sperm DNA fragmentation. Sperm motility was affected by seminal ROS and sperm DNA fragmentation.
检测高活性氧（ROS）精液中精子活力、总抗氧化水平（TAC）、DNA 碎片化和病史。
前瞻性研究。
三级保健医院。
不育男性（n=101）。
I 组（n=57）包括 ROS（<250 相对光单位/秒/×10(6)精子）精液的男性，而 II 组（n=44）包括 ROS（≥250 相对光单位/秒/×10(6)精子）精液的男性。
ROS、TAC、精子 DNA 碎片化、ROS/TAC 评分。
II 组的精子 DNA 碎片化发生率高于 I 组。ROS 水平升高 1.25 的优势比对应于我们患者的 DNA 碎片化增加>10%（95%置信区间 1.01-1.53）。与 I 组相比，II 组显示出较差的活力、更高的白细胞精液症发生率和更高的 ROS-TAC 评分。ROS 与精子曲线速度（r=-.24）、直线性（r=-.24）和精子活力（r=-.31）呈负相关。精子活力与 %TUNEL（+ve）精子呈负相关（r=-.39）。
ROS 水平增加 25%与精子 DNA 碎片化增加 10%相关。ROS 和精子 DNA 碎片化影响精子活力。
Zotero 插件