精英运动员和业余运动男性的精液氧化应激生物标志物与抗氧化剂和精子 DNA 损伤的相关性。

Correlation between seminal oxidative stress biomarkers and antioxidants with sperm DNA damage in elite athletes and recreationally active men.

机构信息

Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Urmia University, Urmia, Iran.

出版信息

Clin J Sport Med. 2012 Mar;22(2):132-9. doi: 10.1097/JSM.0b013e31823f310a.

DOI:10.1097/JSM.0b013e31823f310a

PMID:22246344

Abstract

OBJECTIVE

To evaluate the seminal plasma 8-isoprostane, reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase, total antioxidant capacity (TAC), and levels of sperm DNA fragmentation in elite athletes and recreationally active men.

DESIGN

Prospective design was used for this study.

SETTING

The study was performed in the Exercise Physiology Laboratory of the Urmia University.

PARTICIPANTS

Fifty-six elite athletes and 52 recreationally active men (18-28 years) participated in this study.

INTERVENTION

All subjects had a semen sampling at baseline.

MAIN OUTCOME MEASURES

Total antioxidant capacity and SOD activity were measured by colorimetric assay. Levels of ROS were measured by a chemiluminescence assay. Malondialdehyde levels were measured by thiobarbituric acid reactive substance assay. Catalase activity was measured by monitoring the initial rate of disappearance of hydrogen peroxide. Concentration of free 8-isoprostane was measured by enzyme immunoassay method. Sperm DNA fragmentation was evaluated with the terminal deoxynucleotidyl transferase-mediated fluorescein dUTP nick end-labeling assay.

RESULTS

Recreationally active men have significantly higher levels of body fat, seminal SOD, TAC, and catalase and lower levels of V[Combining Dot Above]O2max, seminal ROS, MDA, and 8-isoprostane and subsequently lower rate of sperm DNA fragmentation when compared with elite athletes (P < 0.001). Significantly negative correlation was observed between sperm DNA fragmentation with body fat, seminal SOD, catalase, and TAC levels (P < 0.001). Significantly positive correlation was observed between sperm DNA fragmentation with V[Combining Dot Above]O2max, seminal 8-isoprostane, ROS, and MDA levels (P < 0.001).

CONCLUSION

Spermatozoa from recreationally active men may be less susceptible to oxidative stress-induced DNA damage and hence infertility.

摘要

目的

评估优秀运动员和休闲运动男性的精液 8-异前列腺素、活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶、总抗氧化能力（TAC）和精子 DNA 碎片化水平。

设计

本研究采用前瞻性设计。

地点

本研究在乌尔米亚大学运动生理学实验室进行。

参与者

56 名优秀运动员和 52 名休闲运动男性（18-28 岁）参加了这项研究。

干预措施

所有受试者在基线时进行精液采样。

主要观察指标

总抗氧化能力和 SOD 活性通过比色法测定。ROS 水平通过化学发光法测定。MDA 水平通过硫代巴比妥酸反应物质测定法测定。过氧化氢酶活性通过监测过氧化氢初始消失率来测定。游离 8-异前列腺素浓度通过酶免疫测定法测定。精子 DNA 碎片化通过末端脱氧核苷酸转移酶介导的荧光素 dUTP 缺口末端标记法评估。

结果

与优秀运动员相比，休闲运动男性的体脂、精液 SOD、TAC 和过氧化氢酶水平显著较高，V[Combining Dot Above]O2max、精液 ROS、MDA 和 8-异前列腺素水平显著较低，精子 DNA 碎片化率也较低（P < 0.001）。精子 DNA 碎片化与体脂、精液 SOD、过氧化氢酶和 TAC 水平呈显著负相关（P < 0.001）。精子 DNA 碎片化与 V[Combining Dot Above]O2max、精液 8-异前列腺素、ROS 和 MDA 水平呈显著正相关（P < 0.001）。