Venkatesh S, Riyaz A M, Shamsi M B, Kumar R, Gupta N P, Mittal S, Malhotra N, Sharma R K, Agarwal A, Dada R
Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.
Andrologia. 2009 Aug;41(4):251-6. doi: 10.1111/j.1439-0272.2009.00943.x.
Reactive oxygen species (ROS) levels in semen are believed to play both physiological and pathological roles in male fertility. The study was aimed to find the clinical significance of ROS levels in infertile Indian men. This pilot study included 33 idiopathic infertile men and 18 proven fertile controls. ROS levels in the washed sperm were measured using chemiluminescence assay and expressed as 10(6) cpm per 20 million spermatozoa. Sperm count, percent sperm motility, and percent normal sperm morphology were found to be significantly (P < 0.0001) reduced in infertile men compared with the controls. Median (minimum, maximum range) ROS levels of the infertile group [24.90 (6.89, 44.71)] were found to be significantly (P < 0.0001) elevated compared with the fertile controls [0.167(0.15, 2.78)]. No significant correlation was seen between ROS levels and semen parameters. Elevated ROS levels in the idiopathic Indian infertile men may be one of the underlying reasons for impaired fertility. Therefore measurement of seminal ROS levels may be used in Indian infertile men for better understanding of the aetiology and selection of antioxidant regimen in the treatment of male infertility. However, large studies may be urgently warranted to find out the role of antioxidants in ROS elevated Indian infertile men through randomised, controlled clinical study.
精液中的活性氧（ROS）水平被认为在男性生育中发挥着生理和病理作用。本研究旨在探寻ROS水平在印度不育男性中的临床意义。这项初步研究纳入了33名特发性不育男性和18名已证实有生育能力的对照者。采用化学发光法测定洗涤后精子中的ROS水平，并表示为每2000万个精子的10(6) 计数每分钟（cpm）。与对照组相比，不育男性的精子计数、精子活力百分比和正常精子形态百分比显著降低（P < 0.0001）。不育组的ROS水平中位数（最小值，最大值范围）[24.90（6.89，44.71）]与有生育能力的对照组[0.167（0.15，2.78）]相比显著升高（P < 0.0001）。ROS水平与精液参数之间未发现显著相关性。印度特发性不育男性中ROS水平升高可能是生育能力受损的潜在原因之一。因此，精液ROS水平的测定可用于印度不育男性，以更好地了解病因并在男性不育治疗中选择抗氧化治疗方案。然而，迫切需要开展大规模研究，通过随机对照临床研究来明确抗氧化剂在ROS水平升高的印度不育男性中的作用。
