[建立适用于可注射骨材料修复的山羊胫骨骨洞缺损模型]
[Establishment of a goat model of tibial bone hole defect suitable for repair using injectable bone materials].
作者信息
Cheng Wen-jun, Jin Dan, Pei Guo-xian, Guo Gang, Li Xu, Xiang Da-yong
机构信息
Department of Orthopedics and Traumatology, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jan;30(1):35-7.
OBJECTIVE
To prepare a goat model of tibial bone hole defect suitable for studies of bone defect repair using tissue-engineered injectable bone materials.
METHODS
A circular hole bone defect 1.2 cm in diameter was induced below the tibial medial plateau of the goat. X-ray, histological inspection, and image analysis were carried out to evaluate the validity of the model in simulating limb bone defect for the study of tissue-engineered injectable bone materials.
RESULTS
At 4 and 8 weeks after the operation, neither X-ray nor histological examination showed obvious bone tissues in the bone defect. Image analysis showed a area of new bone tissue formation of (8.79 - or + 3.63)% in the total defect area at 4 weeks, which increased to (15.41 - or + 4.21)% at 8 weeks.
CONCLUSION
The goat model of tibial bone hole defect established in this study is suitable for studying the ability of injectable bone materials for repairing limb bone defect, and offers a simple and reliable means to simulate the local condition of bone regeneration and mechanical environment of bone defect in the limbs.
目的
制备一种适合使用组织工程可注射骨材料研究骨缺损修复的山羊胫骨骨洞缺损模型。
方法
在山羊胫骨内侧平台下方诱导形成直径1.2 cm的圆形骨洞缺损。进行X线、组织学检查及图像分析，以评估该模型在模拟肢体骨缺损用于组织工程可注射骨材料研究方面的有效性。
结果
术后4周和8周，X线及组织学检查均显示骨缺损处无明显骨组织。图像分析显示，4周时新骨组织形成面积占总缺损面积的(8.79 ± 3.63)%，8周时增加至(15.41 ± 4.21)%。
结论
本研究建立的山羊胫骨骨洞缺损模型适合用于研究可注射骨材料修复肢体骨缺损的能力，为模拟肢体骨再生局部条件及骨缺损力学环境提供了一种简单可靠的方法。
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