迄今为止，在中等酸度沼泽地的厌氧菌和缺氧培养物中发现了未知的[Fe-Fe]-氢化酶基因多样性。
Hitherto unknown [Fe-Fe]-hydrogenase gene diversity in anaerobes and anoxic enrichments from a moderately acidic fen.
机构信息
Department of Ecological Microbiology, University of Bayreuth, 95445 Bayreuth, Germany.
出版信息
Appl Environ Microbiol. 2010 Mar;76(6):2027-31. doi: 10.1128/AEM.02895-09. Epub 2010 Jan 29.
Abstract
Newly designed primers for [Fe-Fe]-hydrogenases indicated that (i) fermenters, acetogens, and undefined species in a fen harbor hitherto unknown hydrogenases and (ii) Clostridium- and Thermosinus-related primary fermenters, as well as secondary fermenters related to sulfate or iron reducers might be responsible for hydrogen production in the fen. Comparative analysis of [Fe-Fe]-hydrogenase and 16S rRNA gene-based phylogenies indicated the presence of homologous multiple hydrogenases per organism and inconsistencies between 16S rRNA gene- and [Fe-Fe]-hydrogenase-based phylogenies, necessitating appropriate qualification of [Fe-Fe]-hydrogenase gene data for diversity analyses.
摘要
新设计的 [Fe-Fe]-氢化酶引物表明：（i）发酵罐、乙酰菌和沼泽中未定义的物种中存在迄今为止未知的氢化酶；（ii）梭菌和 Thermosinus 相关的初级发酵罐，以及与硫酸盐或铁还原剂相关的次级发酵罐可能是沼泽中产生氢气的原因。[Fe-Fe]-氢化酶和 16S rRNA 基因系统发育的比较分析表明，每个生物体中存在同源的多个氢化酶，以及 16S rRNA 基因和 [Fe-Fe]-氢化酶系统发育之间的不一致性，这需要对 [Fe-Fe]-氢化酶基因数据进行适当的多样性分析。
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