Ma Li, Wu Geng, Yang Jian, Huang Liuqin, Phurbu Dorji, Li Wen-Jun, Jiang Hongchen
State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China.
Tibet Plateau Institute of Biology, Lhasa, China.
Front Microbiol. 2021 Jul 21;12:569020. doi: 10.3389/fmicb.2021.569020. eCollection 2021.
Investigating the distribution of hydrogen-producing bacteria (HPB) is of great significance to understanding the source of biological hydrogen production in geothermal environments. Here, we explored the compositions of HPB populations in the sediments of hot springs from the Daggyai, Quzhuomu, Quseyongba, and Moluojiang geothermal zones on the Tibetan Plateau, with the use of Illumina MiSeq high-throughput sequencing of 16S rRNA genes and genes. In the present study, the genes were successfully amplified from the hot springs with a temperature of 46-87°C. The gene phylogenetic analysis showed that the top three phyla of the HPB populations were (14.48%), (14.12%), and (10.45%), while were absent in the top 10 of the HPB populations, although were dominant in the 16S rRNA gene sequences. Canonical correspondence analysis results indicate that the HPB community structure in the studied Tibetan hot springs was correlated with various environmental factors, such as temperature, pH, and elevation. The HPB community structure also showed a spatial distribution pattern; samples from the same area showed similar community structures. Furthermore, one HPB isolate affiliated with was obtained and demonstrated the capacity of hydrogen production. These results are important for us to understand the distribution and function of HPB in hot springs.
研究产氢细菌（HPB）的分布对于理解地热环境中生物产氢的来源具有重要意义。在此，我们利用Illumina MiSeq对16S rRNA基因和相关基因进行高通量测序，探究了青藏高原达格亚、曲卓木、曲色雍巴和莫洛江地热区温泉沉积物中产氢细菌群体的组成。在本研究中，成功从温度为46 - 87°C的温泉中扩增出相关基因。基因系统发育分析表明，产氢细菌群体的前三大门类分别是（14.48%）、（14.12%）和（10.45%），而在产氢细菌群体的前10名中没有出现，尽管在16S rRNA基因序列中占主导地位。典范对应分析结果表明，所研究的西藏温泉中产氢细菌群落结构与温度、pH值和海拔等各种环境因素相关。产氢细菌群落结构也呈现出空间分布模式；来自同一地区的样本显示出相似的群落结构。此外，获得了一株隶属于的产氢细菌分离株，并证明了其产氢能力。这些结果对于我们理解温泉中产氢细菌的分布和功能具有重要意义。
