Boyd Eric S, Spear John R, Peters John W
Department of Chemistry and Biochemistry and Astrobiology, Montana State University, Bozeman, 59717, USA.
Appl Environ Microbiol. 2009 Jul;75(13):4620-3. doi: 10.1128/AEM.00582-09. Epub 2009 May 8.
Degenerate primers for the [FeFe] hydrogenase (hydA) were developed and used in PCRs to examine hydA in microbial mats that inhabit saltern evaporative ponds in Guerrero Negro (GN), Mexico. A diversity of deduced HydA was discovered that revealed unique variants, which may reflect adaptation to the environmental conditions present in GN.
开发了用于[铁铁]氢化酶(hydA)的简并引物,并将其用于聚合酶链反应(PCR)中,以检测墨西哥格雷罗内格罗(GN)盐田蒸发池中微生物垫中的hydA。发现了多种推导的HydA,揭示了独特的变体,这可能反映了对GN存在的环境条件的适应。