Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine, 483 Expo-ro, Yuseong-gu, Daejeon 305-811, Korea.
Biol Pharm Bull. 2010;33(2):329-33. doi: 10.1248/bpb.33.329.
In our ongoing project directed toward the discovery of novel treatments for diabetic complications from herbal medicines, sixteen compounds including three caffeoylquinic acids and four flavonoids were isolated from an EtOAc-soluble extract of the stems and leaves of Erigeron annuus. All the isolates were evaluated in vitro for inhibitory activity on the formation of advanced glycation end products (AGEs) and rat lens aldose reductase (RLAR). Of these, 3,5-di-O-caffeoyl-epi-quinic acid (3) exhibited the most potent inhibitory activity in both the AGEs and aldose reductase (AR) assays. Compound 3 markedly reduced AGEs-bovin serum albumin (BSA) cross-linking in a dose-dependent manner. Furthermore, opacity of lenses was significantly prevented when they were treated with 3 in an ex vivo experiment.
在我们正在进行的项目中，我们从菊科植物飞蓬的茎和叶的乙酸乙酯可溶部分中分离出了十六种化合物，包括三种咖啡酰奎宁酸和四种黄酮类化合物，目的是寻找治疗糖尿病并发症的新草药疗法。所有分离出的化合物都在体外进行了抑制晚期糖基化终产物（AGEs）和大鼠晶状体醛糖还原酶（RLAR）形成的活性评估。在这之中，3,5-二-O-咖啡酰表奎宁酸（3）在AGEs 和醛糖还原酶（AR）测定中均表现出最强的抑制活性。3 号化合物以剂量依赖性方式显著减少 AGEs-牛血清白蛋白（BSA）交联。此外，在离体实验中，当用 3 号化合物处理晶状体时，其混浊度明显降低。
