Division of Human Biology, Faculty of Medicine and Health, International Medical University, Kuala Lumpur, Malaysia.
J Toxicol Sci. 2010 Feb;35(1):41-7. doi: 10.2131/jts.35.41.
Reactive oxygen species (ROS) play an important role in ageing and age-related neurodegenerative changes including Parkinson's disease (PD). PD is characterized by signs of major oxidative stress and mitochondrial damage in the pars compacta of the substantia nigra. Present study was designed to investigate whether the Centella asiatica extract (CAE) would prevent 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in aged Sprague-Dawley rats. Adult, male Sprague-dawley rats of 300-350 g were divided into control, C. asiatica alone, MPTP alone (20 mg/kg, for 21 days) and MPTP with C. asiatica (300 mg/kg for 21 days) groups. Effect of aqueous extract of C. asiatica on oxidative biomarker levels in corpus striatum and hippocampus homogenate was examined. MPTP-challenged rats elicited a significant increase in lipid hydroperoxides (LPO) (p < 0.01), protein-carbonyl-content (PCC) (p < 0.01) and xanthine oxidase (XO) (p < 0.01) when compared with control rats. There was a significant decrease in total antioxidants (TA) (p < 0.001), superoxide dismutase (SOD) (p < 0.001), glutathione peroxidase (GPx) (p < 0.01) and catalase (CAT) (p < 0.001) levels with MPTP treatment. Supplementation of CAE reduced LPO and PCC and significantly increased (p < 0.01) TA and antioxidant enzyme levels (p < 0.01) in corpus striatum and hippocampus. These results show that administration of C. asiatica was effective in protecting the brain against neurodegenerative disorders such as Parkinsonism.
活性氧（ROS）在衰老和与年龄相关的神经退行性变化中起着重要作用，包括帕金森病（PD）。PD 的特征是在黑质致密部有明显的氧化应激和线粒体损伤的迹象。本研究旨在探讨积雪草提取物（CAE）是否能预防 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的老年 Sprague-Dawley 大鼠的神经毒性。成年雄性 Sprague-Dawley 大鼠体重 300-350 g，分为对照组、积雪草提取物组、MPTP 组（20 mg/kg，21 天）和 MPTP 与积雪草提取物组（300 mg/kg，21 天）。检测积雪草提取物对纹状体和海马匀浆氧化生物标志物水平的影响。与对照组相比，MPTP 挑战组大鼠的脂质过氧化物（LPO）（p < 0.01）、蛋白羰基含量（PCC）（p < 0.01）和黄嘌呤氧化酶（XO）（p < 0.01）显著增加。总抗氧化剂（TA）（p < 0.001）、超氧化物歧化酶（SOD）（p < 0.001）、谷胱甘肽过氧化物酶（GPx）（p < 0.01）和过氧化氢酶（CAT）（p < 0.001）水平显著下降。积雪草提取物的补充降低了 LPO 和 PCC，显著增加了 TA 和抗氧化酶水平（p < 0.01），纹状体和海马。这些结果表明，积雪草提取物的给药能有效保护大脑免受帕金森病等神经退行性疾病的影响。
