腹内侧下丘脑损伤后大鼠胰腺全转录组外显子阵列观察到的基因表达分析
Gene Expression Analysis in Rat Pancreas Observed with Whole-Transcript Exon Array after Ventromedial Hypothalamic Lesions.
作者信息
Kiba Takayoshi
机构信息
Division of Modern Medical Technology, Institute for Clinical Research, National Hospital Organization Kure Medical Center and Chugoku Cancer Center, Kure, Japan.
出版信息
Ann Neurosci. 2017 May;24(1):26-31. doi: 10.1159/000464420. Epub 2017 Apr 21.
BACKGROUND
It was reported previously that using Affymetrix Rat Genome 230 2.0, one of a class of standard 3' based arrays, ventromedial hypothalamic (VMH) lesions affected the expressions of cell proliferation-related genes, neuron-related genes, and metabolism-related genes. The released Affymetrix Rat Gene 1.0 ST array has 2 major differences compared with standard 3' based arrays, including Rat Genome 230 2.0: it interrogates the entire mRNA transcript and uses DNA targets.
PURPOSE
This study is aimed at assessing the impact of these differences on the array performance.
METHODS
The study used Rat Gene 1.0 ST array, one of a class of whole-transcript rat exon arrays to examine the cellular mechanisms of gene regulation in the rat pancreas after VMH lesions.
RESULTS
Although the results showed that VMH lesions regulated genes involved in enzymes, metabolism, transport, binding differentiation, migration, morphology, apoptosis, neuron and immunity, the probes identified by these 2 arrays were remarkably different.
CONCLUSION
This study also confirmed that VMH lesions may affect the expression of many functional genes in rat pancreas.
背景
先前有报道称，使用一类基于3'端的标准阵列之一的Affymetrix大鼠基因组230 2.0芯片，腹内侧下丘脑（VMH）损伤会影响细胞增殖相关基因、神经元相关基因和代谢相关基因的表达。已发布的Affymetrix大鼠基因1.0 ST芯片与基于3'端的标准阵列（包括大鼠基因组230 2.0）相比有两个主要差异：它能检测整个mRNA转录本并使用DNA靶点。
目的
本研究旨在评估这些差异对阵列性能的影响。
方法
本研究使用大鼠基因1.0 ST芯片，这是一类全转录本大鼠外显子芯片，用于研究VMH损伤后大鼠胰腺中基因调控的细胞机制。
结果
尽管结果显示VMH损伤调控了参与酶、代谢、转运、结合、分化、迁移、形态、凋亡、神经元和免疫的基因，但这两种芯片鉴定出的探针有显著差异。
结论
本研究还证实VMH损伤可能影响大鼠胰腺中许多功能基因的表达。
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