Tsinghua University, Beijing, China.
Acta Biochim Biophys Sin (Shanghai). 2010 Feb;42(2):91-7. doi: 10.1093/abbs/gmp111.
Trichosanthin (TCS) is a type I ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus type 1, and has been clinically applied in acquired immunodeficiency syndrome (AIDS) therapy. Previous studies revealed that TCS recognized human immunodeficiency virus type 1 (HIV-1) particles. Here, we investigated the physical relationship between TCS and HIV-1 particles, and demonstrated that TCS penetrates into viral particles, where it is protected from various protease digestion. The penetration of TCS exerts no obvious effect on viral integrity. FYY140-142, D176, and K177 were identified as key amino acid residues for the membranetranslocation process. Moreover, TCS penetrated into HIV-1 virions showed potent anti-viral activity. Overall, the observations suggest that the penetration of TCS into HIV-1 particles may be important for eliminating the virus.
天花粉蛋白（TCS）是一种 I 型核糖体失活蛋白，对人类免疫缺陷病毒 1 （HIV-1）具有强烈的抑制活性，已在获得性免疫缺陷综合征（AIDS）治疗中临床应用。先前的研究表明，TCS 识别人类免疫缺陷病毒 1 （HIV-1）颗粒。在这里，我们研究了 TCS 与 HIV-1 颗粒之间的物理关系，并证明 TCS 穿透病毒颗粒，在那里它受到各种蛋白酶消化的保护。TCS 的穿透对病毒完整性没有明显影响。鉴定出 FYY140-142、D176 和 K177 是跨膜转运过程的关键氨基酸残基。此外，穿透到 HIV-1 病毒粒子中的 TCS 表现出强大的抗病毒活性。总的来说，这些观察结果表明，TCS 穿透 HIV-1 颗粒可能对消除病毒很重要。
