Department of Anatomy and Neurobiology, MN-225 Chandler Medical Center, University of Kentucky College of Medicine, Lexington, KY 40536-0298, USA.
Neurochem Res. 2010 May;35(5):727-34. doi: 10.1007/s11064-010-0128-0. Epub 2010 Jan 30.
Neurturin (NTN) is a member of the glial cell line-derived neurotrophic factor (GDNF) family; and, while GDNF has been shown to increase dopamine (DA) release in normal animals, the ability of NTN to alter DA release has not been previously reported. The purpose of the present study was to determine if NTN could alter striatal DA release, and to compare the effects of NTN to GDNF. Male Fischer-344 rats were given a single injection of vehicle or 5 microg NTN or GDNF into the right substantia nigra. Three weeks later microdialysis experiments were conducted to assess striatal DA release. Basal extracellular levels of striatal DA were not affected by either NTN or GDNF. However, both NTN and GDNF led to increases in amphetamine-evoked overflow of DA from the ipsilateral striatum, and there was a trend for potassium-evoked overflow to be augmented. Postmortem tissue levels of DA were decreased by approximately 20% in the striatum, and increased by approximately 100% in the substantia nigra, on the ipsilateral side of the brain compared to the contralateral side following both NTN and GDNF injection. Thus, NTN, like GDNF, can augment striatal DA release, and the magnitude of the NTN effects are similar to those of GDNF.
神经营养因子（NTN）是胶质细胞系衍生的神经营养因子（GDNF）家族的成员；虽然 GDNF 已被证明可以增加正常动物的多巴胺（DA）释放，但 NTN 改变 DA 释放的能力尚未得到报道。本研究的目的是确定 NTN 是否可以改变纹状体 DA 的释放，并比较 NTN 和 GDNF 的作用。雄性 Fischer-344 大鼠单侧脑室内注射载体或 5μg NTN 或 GDNF。3 周后进行微透析实验以评估纹状体 DA 的释放。NTN 或 GDNF 均未影响纹状体 DA 的基础细胞外水平。然而，NTN 和 GDNF 均导致对侧纹状体中 DA 诱导的溢出增加，并且钾诱导的溢出有增加的趋势。与脑的对侧相比，NTN 和 GDNF 注射后，同侧纹状体中的 DA 组织水平降低了约 20%，而黑质中的 DA 水平增加了约 100%。因此，NTN 与 GDNF 一样，可以增强纹状体 DA 的释放，并且 NTN 的作用大小与 GDNF 相似。
