Physical Chemistry, Institute of Chemistry, Martin Luther University Halle-Wittenberg, Halle (Saale) D-06099, Germany.
J Biomed Mater Res B Appl Biomater. 2010 Apr;93(1):275-84. doi: 10.1002/jbm.b.31585.
The fate of poly(vinyl alcohol) (PVA, 195,000 g/mol) was studied in rabbits and nude mice after intraperitoneal (i.p.) administration. In-vivo fluorescence imaging using nude mice allowed for studies of tetramethylrhodamine labeled PVA distribution in the body and tracking the urinary excretion. The excreted PVA was studied in detail after collecting the urine of rabbits over a time period of 28 days. The PVA was separated from the urine by dialysis and analyzed by FTIR spectroscopy, (1)H-NMR spectroscopy, and size exclusion chromatography (SEC). Even after extensive dialysis, it was found that the excreted PVA showed a characteristic brownish color. The spectroscopic techniques revealed that this color was caused by the urine pigment (a metabolite of bilirubin) that could not be separated completely from the PVA. SEC showed unambiguously that the PVA with the very high molar mass had a glomerular permeability in the kidneys. Simultaneously, histological studies of the kidneys and the liver demonstrated that the tissues did not show any obvious damage.
聚（聚乙烯醇）（PVA，195,000 g/mol）在腹腔内（i.p.）给药后的兔子和裸鼠中的命运进行了研究。使用裸鼠进行体内荧光成像，可研究四甲基罗丹明标记的 PVA 在体内的分布并跟踪尿液排泄。在收集兔子尿液超过 28 天的时间后，详细研究了排泄的 PVA。通过透析将 PVA 从尿液中分离出来，并通过傅里叶变换红外光谱（FTIR）、（1）H-NMR 光谱和尺寸排阻色谱（SEC）进行分析。即使经过广泛的透析，也发现排泄的 PVA 呈现出特征性的棕色。光谱技术表明，这种颜色是由尿液色素（胆红素的代谢物）引起的，无法从 PVA 中完全分离。SEC 明确表明，具有非常高摩尔质量的 PVA 具有肾脏中的肾小球通透性。同时，对肾脏和肝脏的组织学研究表明，组织没有显示出任何明显的损伤。
