Chen Xiujie, Liu Lei, Sun Zhizhong, Liu Yongsheng, Xu Jiankai, Liu Sibo, Huang Bangqing, Ma Ling, Yu Zhiguo, Bi Kaishun
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Biomed Chromatogr. 2010 Aug;24(8):826-32. doi: 10.1002/bmc.1370.
Accurate and reproducible HPLC methods were developed and validated for the determination of concentrations of luteolin (LT) and tetra-acetyl-luteolin (TALT) in rat plasma. HPLC analyses were performed on an Agilent TC-C(18) column protected by a guard Agilent Zorbax Eclipse Plus. The mobile phase for LT was a binary mixture of acetonitrile-water (40:60, v/v) containing 0.5% phosphoric acid at a flow rate of 1.0 mL/min, and that for TALT was a binary mixture of methanol-water (70 : 30, v/v) containing 0.5% glacial acetic acid at the same flow rate. The UV detection wavelength for both analytes was set at 350 nm. The calibration curve was linear over the range of 40-1800 ng/mL, the lower limit of quantitation was 40 ng/mL and the lower limit of detection was 20 ng/mL for both LT and TALT. The intra- and inter-day precision (RSD) values for all samples were within 7.9%. The concentration-time curves of LT and TALT after oral administration (30 mg/kg) were both fitted to a two-compartment model. The pharmacokinetic characteristics of TALT were better than that of LT in the maximum plasma concentration (C(max)) and the area under the concentration-time curve (AUC).
建立并验证了准确且可重复的高效液相色谱（HPLC）方法，用于测定大鼠血浆中木犀草素（LT）和四乙酰木犀草素（TALT）的浓度。HPLC分析在配备安捷伦Zorbax Eclipse Plus保护柱的安捷伦TC-C(18)柱上进行。LT的流动相为含0.5%磷酸的乙腈-水二元混合物（40:60，v/v），流速为1.0 mL/min；TALT的流动相为含0.5%冰醋酸的甲醇-水二元混合物（70:30，v/v），流速相同。两种分析物的紫外检测波长均设定为350 nm。校准曲线在40 - 1800 ng/mL范围内呈线性，LT和TALT的定量下限均为40 ng/mL，检测下限均为20 ng/mL。所有样品的日内和日间精密度（相对标准偏差，RSD）值均在7.9%以内。口服给药（30 mg/kg）后LT和TALT的浓度-时间曲线均符合二室模型。TALT在最大血浆浓度（C(max)）和浓度-时间曲线下面积（AUC）方面的药代动力学特征优于LT。
