糙叶败酱草（L.f.）的细胞毒性潜力和高效液相色谱法测定木樨草素含量。

Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC.

机构信息

Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576 104, India.

出版信息

BMC Complement Altern Med. 2014 Oct 28;14:421. doi: 10.1186/1472-6882-14-421.

DOI:10.1186/1472-6882-14-421

PMID:25348394

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4226858/

Abstract

BACKGROUND

Anisochilus carnosus (L.f.) wall (Lamiaceae), an annual herb which grows at high altitude is used extensively in folk medicine for the treatment of ailments such as gastric ulcer and skin diseases. The aim of our study was to evaluate the anticancer activity of different extracts of the leaves of A.carnosus. An attempt was also made to estimate the luteolin content in different extracts of Anisochilus carnosus by HPLC (High Performance Liquid Chromatography).

METHODS

In the current study, we explored the cytotoxic potential of petroleum ether, ethanolic and aqueous extracts of A.carnosus against breast adenocarcinoma cell line (BT-549), by in vitro MTT and SRB assay. We also detected the luteolin content in different extracts (ethanolic and aqueous) of A.carnosus by using HPLC as a tool of analysis.

RESULTS

The results demonstrate that petroleum ether and ethanolic extract of A.carnosus showed potent cytotoxic effect against BT-549 with an IC50 of 22.5 μg/ml (petroleum ether extract) and 87.24 μg/ml (ethanolic extract), by SRB assay, and 18.35 μg/ml (petroleum ether extract) and 58.64 μg/ml (ethanolic extract), by MTT assay. The aqueous extracts showed less cytotoxic effect with an IC50 of 211.26 μg/ml (by SRB assay) and 238.91 μg/ml (by MTT assay). HPLC results of luteolin content in various extracts using luteolin as the marker compound indicated the ethanol extract to contain the highest concentration of luteolin (0.372% w/w). The aqueous extract contained lower concentration of luteolin (0.282% w/w).

CONCLUSION

Our findings demonstrate that petroleum ether and ethanolic extract of A.carnosus shows promising anticancer activity and has the potential to be developed into a therapeutic option for the treatment of cancer.

摘要

背景

肉叶金腰（唇形科）是一种生长在高海拔地区的一年生草本植物，在民间医学中被广泛用于治疗胃溃疡和皮肤病等疾病。我们的研究旨在评估肉叶金腰叶的不同提取物的抗癌活性。我们还尝试通过 HPLC（高效液相色谱法）来估计不同提取物中的木犀草素含量。

方法

在目前的研究中，我们通过体外 MTT 和 SRB 测定法，探索了石油醚、乙醇和水提取物对乳腺癌腺癌细胞系（BT-549）的细胞毒性潜力。我们还使用 HPLC 作为分析工具检测了肉叶金腰不同提取物（乙醇和水）中的木犀草素含量。

结果

结果表明，石油醚和乙醇提取物对 BT-549 表现出强烈的细胞毒性作用，SRB 测定法的 IC50 分别为 22.5μg/ml（石油醚提取物）和 87.24μg/ml（乙醇提取物），MTT 测定法的 IC50 分别为 18.35μg/ml（石油醚提取物）和 58.64μg/ml（乙醇提取物）。水提取物的细胞毒性作用较弱，SRB 测定法的 IC50 为 211.26μg/ml，MTT 测定法的 IC50 为 238.91μg/ml。使用木犀草素作为标记化合物的各种提取物中木犀草素含量的 HPLC 结果表明，乙醇提取物含有最高浓度的木犀草素（0.372%w/w）。水提取物含有较低浓度的木犀草素（0.282%w/w）。