Fraternale Daniele, Giamperi Laura, Bucchini Anahi, Sestili Piero, Paolillo Marco, Ricci Donata
Dipartimento di Scienze dell'Uomo, dell'Ambiente e della Natura, Sez. Biologia Vegetale, Università degli Studi di Urbino "Carlo Bo ", Via Bramante, 28 - 61029, Urbino, PU, Italy.
Nat Prod Commun. 2009 Dec;4(12):1665-70.
The antioxidant activity was assessed of fresh juice from Prunus spinosa L. fruit (Rosaceae) growing wild in Urbino (central Italy) by using different cell-free in vitro analytical methods: 5-lipoxygenase test, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and oxygen radical absorbance capacity (ORAC). Trolox was used as the reference antioxidant compound. In the 5-lipoxygenase and DPPH tests the fresh fruit juice of P. spinosa showed good antioxidant activity when compared with Trolox, while the ORAC value was 36.0 micromol eq. Trolox/g of fruit. These values are in accord with data reported in the literature for small fruits such as Vaccinium, Rubus and Ribes. The antioxidant capacity in cell-free systems of P. spinosa juice has been compared with its cytoprotective - bona fide antioxidant activity in cultured human promonocytes (U937 cells) exposed to hydrogen peroxide. The antioxidant activity of red berries has been correlated with their anthocyanin content. The results of this study indicate that the three most representative anthocyanins in P. spinosa fruit juice (cyanidin-3-rutinoside, peonidin-3-rutinoside and cyanidin-3-glucoside) are likely to play an important role in its antioxidant properties.
5-脂氧合酶测试、1,1-二苯基-2-苦基肼（DPPH）自由基清除法和氧自由基吸收能力（ORAC），对生长在意大利中部乌尔比诺的野生李属刺李（蔷薇科）果实的新鲜果汁的抗氧化活性进行了评估。使用Trolox作为参考抗氧化化合物。在5-脂氧合酶和DPPH测试中，与Trolox相比，刺李的新鲜果汁显示出良好的抗氧化活性，而ORAC值为36.0微摩尔当量Trolox/克果实。这些值与文献中报道的越橘、悬钩子和醋栗等小果实的数据一致。将刺李果汁在无细胞系统中的抗氧化能力与其在暴露于过氧化氢的培养人原单核细胞（U937细胞）中的细胞保护——真正的抗氧化活性进行了比较。红色浆果的抗氧化活性与其花青素含量相关。本研究结果表明，刺李果汁中三种最具代表性的花青素（矢车菊素-3-芸香糖苷、芍药素-3-芸香糖苷和矢车菊素-3-葡萄糖苷）可能在其抗氧化特性中发挥重要作用。
