绒毛补血草（Anthriscifolia变种）精油的化学成分及抗分枝杆菌活性

Chemical composition and antimycobacterial activity of the essential oil from Anemia tomentosa var. anthriscifolia.

作者信息

Pinto Shaft Corrêa, Leitão Gilda Guimarães, de Oliveira Danilo Ribeiro, Bizzo Humberto Ribeiro, Ramos Daniela Fernandes, Coelho Tatiane Silveira, Silva Pedro Eduardo A, Lourenço Maria Cristina S, Leitão Suzana Guimarães

机构信息

Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, CCS, Bloco H, Ilha do Fundão, 21941-590, Rio de Janeiro, Brazil.

出版信息

Nat Prod Commun. 2009 Dec;4(12):1675-8.

PMID:20120105

Abstract

The essential oil from Anemia tomentosa var. anthriscifolia showed in vitro activity against Mycobacterium tuberculosis (MIC 100 microg/ml) and therefore was characterized by gas chromatography (GC) and by gas chromatography coupled with mass spectrometry (GC-MS). The major constituents of this essential oil were triquinane sesquiterpenes: silphiperfol-6-ene (14.7%), (-)-epi-presilphiperfolan-1-ol (30.6%), presilphiperfol-7-ene (3.9%), cameroonan-7 alpha-ol (4.4%), prenopsan-8-ol (1.9%) and presilphiperfolan-8-ol (8.3%), suggesting the existence of different chemotypes for this species. The essential oil was fractionated by column chromatography and its major constituent and fractions were assayed against Mycobacterium tuberculosis and M. smegmatis. (-)-epi-Presilphiperfolan-1-ol exhibited an MIC of 120 microg/ml against M. tuberculosis H37Rv.

摘要

绒毛绵毛菊（Anemia tomentosa var. anthriscifolia）的精油在体外显示出对结核分枝杆菌的活性（最低抑菌浓度为100微克/毫升），因此通过气相色谱（GC）以及气相色谱-质谱联用（GC-MS）对其进行了表征。该精油的主要成分是三环倍半萜：蛇床烯-6-烯（14.7%）、（-）-表前蛇床子醇（30.6%）、前蛇床烯-7-烯（3.9%）、卡麦罗烷-7α-醇（4.4%）、前诺普桑-8-醇（1.9%）和前蛇床子醇-8-醇（8.3%），这表明该物种存在不同的化学型。通过柱色谱对精油进行了分离，并对其主要成分和馏分针对结核分枝杆菌和耻垢分枝杆菌进行了检测。（-）-表前蛇床子醇对结核分枝杆菌H37Rv的最低抑菌浓度为120微克/毫升。