Duarte Marta Cristina Teixeira, Leme Ewerton Eduardo, Delarmelina Camila, Soares Andressa Almeida, Figueira Glyn Mara, Sartoratto Adilson
Research Center for Chemistry, Biology and Agriculture, State University of Campinas, P.O. Box 6171, CEP 13083-970 Campinas, SP, Brazil.
J Ethnopharmacol. 2007 May 4;111(2):197-201. doi: 10.1016/j.jep.2006.11.034. Epub 2006 Dec 3.
Essential oils obtained from leaves of 29 medicinal plants commonly used in Brazil were screened against 13 different Escherichia coli serotypes. The oils were obtained by water-distillation using a Clevenger-type system and their minimal inhibitory concentration (MIC) were determined by microdilution method. Essential oil from Cymbopogon martinii exhibited a broad inhibition spectrum, presenting strong activity (MIC between 100 and 500 microg/mL) against 10 out of 13 Escherichia coli serotypes: three enterotoxigenic, two enteropathogenic, three enteroinvasive and two shiga-toxin producers. C. winterianus inhibited strongly two enterotoxigenic, one enteropathogenic, one enteroinvasive and one shiga-toxin producer serotypes. Aloysia triphylla also shows good potential to kill Escherichia coli with moderate to strong inhibition. Other essential oils showed antimicrobial properties, however with a more restricted action against the serotypes studied. Chemical analysis of Cymbopogon martinii essential oil performed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS) showed the presence of compounds with known antimicrobial activity, including geraniol, geranyl acetate and trans-cariophyllene, which tested separately, indicated geraniol as antimicrobial active compound. The significant antibacterial activity of Cymbopogon martinii oil suggests that they could serve as a source for compounds with therapeutic potential.
对从巴西常用的29种药用植物叶片中提取的香精油针对13种不同的大肠杆菌血清型进行了筛选。这些香精油采用克利夫enger型系统通过水蒸馏法获得，其最低抑菌浓度（MIC）通过微量稀释法测定。马丁香茅的香精油呈现出广泛的抑制谱，对13种大肠杆菌血清型中的10种表现出强活性（MIC在100至500微克/毫升之间）：三种产肠毒素型、两种致病性、三种侵袭性和两种产志贺毒素型。爪哇香茅强烈抑制两种产肠毒素型、一种致病性、一种侵袭性和一种产志贺毒素型血清型。三裂叶排草对杀灭大肠杆菌也显示出良好的潜力，具有中度至强抑制作用。其他香精油也显示出抗菌特性，不过对所研究的血清型作用较为有限。通过气相色谱（GC）和气相色谱/质谱联用（GC-MS）对马丁香茅香精油进行的化学分析表明，其含有具有已知抗菌活性的化合物，包括香叶醇、乙酸香叶酯和反式石竹烯，单独测试表明香叶醇是抗菌活性化合物。马丁香茅油的显著抗菌活性表明，它们可作为具有治疗潜力化合物的来源。
