El-Marhoumy Sirria M, El-Nouby Kholoud A, Emara Mohamed A, Abou Rayia Dina M
Department of Parasitology, Faculty of Medicine, Tanta University, Egypt.
J Egypt Soc Parasitol. 2009 Dec;39(3):917-32.
This study assessed the effectiveness of autoclaved cercarial vaccine (ACV) in protection against Schistosoma mansoni infection in 125 Swiss albino mice classified into two main groups: GI: a control group. GII: a test vaccinated with ACV, in a single dose of 0.1 ml of 10(4) ml ACV (G.IIa), double dose; 0.2ml (G.IIb) and two single doses 2 weeks apart (G.IIc). Four weeks later, all mice were challenged with S. mansoni cercariae and sacrificed 10 weeks post infection (P.I.). The results revealed that the vaccine in a single dose (G.IIa) induced a high level of protection against S. mansoni infection. There was a significant reduction in the mean number of adult worm (91.12%), ova/gram liver (91.87%), ova/gram intestine (89.09%) and number & size of granulomas in liver (92.92% & 43.53% respectively). Besides, ACV induced a significant increase in the level of IL-10 mRNA expression as compared to the control group.
本研究评估了高压灭菌尾蚴疫苗（ACV）对125只瑞士白化小鼠曼氏血吸虫感染的预防效果，这些小鼠分为两个主要组：GI：对照组。GII：用ACV进行疫苗接种的试验组，单剂量为0.1 ml含10(4)个/ml的ACV（G.IIa），双剂量为0.2 ml（G.IIb），以及间隔2周的两个单剂量（G.IIc）。四周后，所有小鼠均用曼氏血吸虫尾蚴攻击，并在感染后10周处死。结果显示，单剂量疫苗（G.IIa）对曼氏血吸虫感染诱导了高水平的保护。成虫平均数量（91.12%）、肝脏每克虫卵数（91.87%）、肠道每克虫卵数（89.09%）以及肝脏肉芽肿数量和大小（分别为92.92%和43.53%）均显著减少。此外，与对照组相比，ACV诱导IL-10 mRNA表达水平显著增加。
