Nath Kamlesh, Singh Dharam, Shyam Shilpa, Sharma Y K
Laboratory of Environmental Sciences, Department of Botany, University of Lucknow, Lucknow, India.
J Environ Biol. 2009 Mar;30(2):227-34.
The various dilution levels of treated tannery effluent (T.E.) (10, 25, 50 and 100%) and Cr6+ (0.5, 2.0, 5.0 and 10 ppm) were used in experiment to know their effect on seed germination, seedling growth, pigments and enzymes content in Black gram (Phaseolus mungo Roxb.). Chromium is known as the main toxic component of tannery effluent so its various concentration were given to know their effects. For the recovery of plant damage, protective value of 10 and 25 ppm of zinc, potassium and iron sulphate were also given with 50% treated tannery effluent and 10 ppm Cr6+ levels in separate petridishes. The different concentrations of tannery effluent and Cr6+ showed significant reduction in germination percentage, seedling growth (plumule and radicle length, number of lateral roots, fresh and dry weight, and moisture %) and pigments (chlorophyll, pheophytin and carotenoids) with increase in concentrations. The lower doses of tannery effluent (10%) and Cr6+ (0.5, 2 and 5 ppm) slightly increases the pigments concentration. The amylase activity and total sugar contents were also significantly decreased while catalase and peroxidase activity showed significant increase with rise in concentrations of treatments. The lower concentrations of tannery effluent (dilution 10 and 25%) and Cr6+ (0.5 and 2 ppm) showed significant increase in total protein contents while decrease at higher concentrations. The zinc, potassium and iron treatments led to recover the damage caused by chromium and tannery effluent in all parameters. In recovery treatments zinc showed highest and significant recovery in maximum parameters. Iron also showed almost similar effect to the zinc while potassium showed minimum recovery.
采用不同稀释水平的制革废水（10%、25%、50%和100%）和不同浓度的六价铬（0.5、2.0、5.0和10 ppm）进行实验，以了解它们对黑绿豆（绿豆）种子萌发、幼苗生长、色素和酶含量的影响。铬是制革废水中的主要有毒成分，因此设置其不同浓度来了解其影响。为了恢复植物损伤，在单独的培养皿中，还对50%的制革废水和10 ppm六价铬水平添加了10和25 ppm的硫酸锌、硫酸钾和硫酸铁进行保护。不同浓度的制革废水和六价铬随着浓度增加，种子发芽率、幼苗生长（胚轴和胚根长度、侧根数、鲜重和干重以及含水量）和色素（叶绿素、脱镁叶绿素和类胡萝卜素）显著降低。较低剂量的制革废水（10%）和六价铬（0.5、2和5 ppm）略微增加了色素浓度。淀粉酶活性和总糖含量也显著降低，而过氧化氢酶和过氧化物酶活性随着处理浓度的升高而显著增加。较低浓度的制革废水（稀释10%和25%）和六价铬（0.5和2 ppm）总蛋白含量显著增加，而在较高浓度下降低。锌、钾和铁处理使铬和制革废水对所有参数造成的损伤得到恢复。在恢复处理中，锌在大多数参数上显示出最高且显著的恢复效果。铁的效果与锌几乎相似，而钾的恢复效果最小。
