Department of Experimental Diagnostic Imaging, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
ACS Nano. 2010 Feb 23;4(2):1033-41. doi: 10.1021/nn901181c.
We report dual-functional hollow gold nanospheres (HAuNS, approximately 40-nm diameter) capable of mediating both photothermal ablation of cancer cells and drug release upon near-infrared (NIR) light irradiation. As high as 63% DOX by weight ( approximately 1.7 microg DOX/microg Au) could be loaded to polyethylene glycol (PEG)-coated HAuNS since DOX was coated to both the outer and the inner surfaces of HAuNS. Irradiation with NIR laser induced photothermal conversion, which triggered rapid DOX release from DOX-loaded HAuNS. The release of DOX was also pH-dependent, with more DOX released in aqueous solution at lower pH. Significantly greater cell killing was observed when MDA-MB-231 cells incubated with DOX-loaded HAuNS were irradiated with NIR light, attributable to both HAuNS-mediated photothermal ablation and cytotoxicity of released free DOX.
我们报告了一种双重功能的空心金纳米球（HAuNS，直径约 40nm），能够在近红外（NIR）光照射下介导癌细胞的光热消融和药物释放。由于 DOX 被包覆在 HAuNS 的内外表面上，因此可以将高达 63%的 DOX 重量（约 1.7μg DOX/mg Au）负载到聚乙二醇（PEG）包覆的 HAuNS 上。用 NIR 激光照射会引发光热转换，从而迅速从负载 DOX 的 HAuNS 中释放 DOX。DOX 的释放也依赖于 pH 值，在较低 pH 值的水溶液中释放更多的 DOX。当用 NIR 光照射与负载 DOX 的 HAuNS 孵育的 MDA-MB-231 细胞时，观察到明显更高的细胞杀伤率，这归因于 HAuNS 介导的光热消融和释放的游离 DOX 的细胞毒性。
