Meyers Marianne, Stoffels Charlotte B A, Frache Gilles, Letellier Elisabeth, Feucherolles Maureen
Department of Life Sciences and Medicine, Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg.
Front Cell Infect Microbiol. 2025 Jun 4;15:1559870. doi: 10.3389/fcimb.2025.1559870. eCollection 2025.
The role of the microbiome in cancer metastasis has emerged as a critical area of research, with growing evidence suggesting that microbial composition and interactions within the tumour microenvironment may significantly influence metastatic progression. This review explores the role of the microbiome in cancer metastasis, as well as potential key bacteria and their mechanisms through which they could impact tumour dissemination, seeding and growth. Biological models used to study metastasis are discussed to provide context for the further investigation of these interactions. In order to answer unresolved questions regarding the microbiome's involvement in metastatic dissemination, recent advancements in spatial biology techniques are examined, including spatial genomics, transcriptomics, proteomics and metabolomics, which enable the spatial mapping of microbial interactions within the tumour microenvironment. Additionally, multimodal-omics imaging approaches are highlighted for their potential to integrate multiple molecular layers, offering comprehensive insights into the microbiome's role in cancer metastasis. The review also addresses the challenges and limitations of these techniques, underscoring the complexity of studying microbiome-tumour interactions and offering directions for future research to better explore and target the microbiological landscape in metastatic cancer.
微生物群落在癌症转移中的作用已成为一个关键的研究领域，越来越多的证据表明，肿瘤微环境中的微生物组成和相互作用可能会显著影响转移进程。本综述探讨了微生物群落在癌症转移中的作用，以及潜在的关键细菌及其可能影响肿瘤播散、定植和生长的机制。文中讨论了用于研究转移的生物学模型，以便为进一步研究这些相互作用提供背景。为了回答有关微生物群落参与转移播散的未解决问题，本文研究了空间生物学技术的最新进展，包括空间基因组学、转录组学、蛋白质组学和代谢组学，这些技术能够对肿瘤微环境内的微生物相互作用进行空间映射。此外，多组学成像方法因其整合多个分子层面的潜力而受到关注，它能全面洞察微生物群落在癌症转移中的作用。本综述还讨论了这些技术面临的挑战和局限性，强调了研究微生物群落与肿瘤相互作用的复杂性，并为未来研究提供了方向，以便更好地探索和靶向转移性癌症中的微生物格局。
