Department of Medical Microbiology, Radboud University Nijmegen Medical Centre and Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands.
Viral Immunol. 2010 Feb;23(1):99-104. doi: 10.1089/vim.2009.0072.
Previous studies have shown that enteroviral RNA can be detected in blood at the onset of type 1 diabetes (T1D). The infection may play a role in triggering T1D and genetic host factors may contribute to this process. We investigated (1) whether enterovirus is present at the onset of T1D in peripheral blood mononuclear cells (PBMC), plasma, throat, or stool, and (2) whether enteroviral presence is linked with HLA-DR type and/or polymorphisms in melanoma differentiation-associated gene 5 (MDA5) and 2'-5' oligoadenylate synthetase 1 (OAS1), factors of antiviral immunity. To this end, PBMC, plasma, throat, and stool samples from 10 T1D patients and 20 unrelated controls were tested for the presence of enteroviruses (RT-PCR), for HLA-DR type, and polymorphisms in MDA5 and OAS1. Enterovirus RNA was detected in PBMC of 4/10 T1D patients, but none of 20 controls. Plasma was positive in 2/10 T1D patients and none of 20 controls, suggesting that enteroviruses found at the onset of T1D are mainly present in PBMC. All throat samples from positive T1D patients were virus-negative and only 1 fecal sample was positive. The negative results for all throat and most stool samples argues against acute infection. Enterovirus presence was linked with HLA-DR4, but not with polymorphisms in MDA5 or OAS1.
先前的研究表明，肠道病毒 RNA 可在 1 型糖尿病（T1D）发病时在血液中检测到。感染可能在引发 T1D 中发挥作用，并且遗传宿主因素可能促成这一过程。我们研究了（1）肠道病毒是否存在于 T1D 患者外周血单核细胞（PBMC）、血浆、咽喉或粪便中，以及（2）肠道病毒的存在是否与 HLA-DR 类型以及黑色素瘤分化相关基因 5（MDA5）和 2'-5'寡聚腺苷酸合成酶 1（OAS1）的多态性相关，这些都是抗病毒免疫的因素。为此，我们对 10 名 T1D 患者和 20 名无关对照的 PBMC、血浆、咽喉和粪便样本进行了肠道病毒（RT-PCR）检测、HLA-DR 类型检测以及 MDA5 和 OAS1 的多态性检测。在 4/10 的 T1D 患者的 PBMC 中检测到肠道病毒 RNA，但在 20 名对照中均未检测到。在 2/10 的 T1D 患者的血浆中检测到阳性，但在 20 名对照中均未检测到，这表明在 T1D 发病时发现的肠道病毒主要存在于 PBMC 中。所有阳性 T1D 患者的咽喉样本均为病毒阴性，只有 1 份粪便样本为阳性。所有咽喉和大部分粪便样本的阴性结果表明不存在急性感染。肠道病毒的存在与 HLA-DR4 相关，但与 MDA5 或 OAS1 的多态性无关。
