Department of Physiology & Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Acta Physiol (Oxf). 2010 Jun;199(2):221-30. doi: 10.1111/j.1748-1716.2010.02091.x. Epub 2010 Feb 1.
To examine the phenotype of mice that lack the adenosine A(3) receptor (A(3)R).
We examined the heart rate, body temperature and locomotion continuously by telemetry over several days. In addition, the effect of the adenosine analogue R-N(6)-phenylisopropyl-adenosine (R-PIA) was examined. We also examined heat production and food intake.
We found that the marked diurnal variation in activity, heart rate and body temperature, with markedly higher values at night than during day time, was reduced in the A(3)R knock-out mice. Surprisingly, the reduction in heart rate, activity and body temperature seen after injection of R-PIA in wild type mice was virtually eliminated in the A(3)R knock-out mice. The marked reduction in activity was associated with a decreased heat production, as expected. However, the A(3)R knock-out mice, surprisingly, had a higher food intake but no difference in body weight compared to wild type mice.
The mice lacking adenosine A(3) receptors exhibit a surprisingly clear phenotype with changes in diurnal rhythm and temperature regulation. Whether these effects are due to a physiological role of A(3) receptors in these processes or whether they represent a role in development remains to be elucidated.
研究缺乏腺苷 A（3）受体（A（3）R）的小鼠表型。
我们通过遥测技术连续几天监测心率、体温和运动情况。此外，还检测了腺苷类似物 R-N（6）-苯异丙基-腺苷（R-PIA）的作用。我们还检测了产热和食物摄入情况。
我们发现，A（3）R 敲除小鼠的活动、心率和体温的昼夜变化明显减少，夜间的数值明显高于白天。令人惊讶的是，在野生型小鼠中注射 R-PIA 后观察到的心率、活动和体温的明显下降，在 A（3）R 敲除小鼠中几乎完全消除。如预期的那样，活动的明显减少与产热减少有关。然而，令人惊讶的是，A（3）R 敲除小鼠的食物摄入量增加，但体重与野生型小鼠无差异。
缺乏腺苷 A（3）受体的小鼠表现出明显的表型变化，包括昼夜节律和体温调节的变化。这些影响是由于 A（3）受体在这些过程中的生理作用，还是它们代表了在发育过程中的作用，仍有待阐明。
