"Petru Poni" Institute of Macromolecular Chemistry, 41A Grigore Ghica Voda Alley, 700487 Iasi, Romania.
Department of Microbiology, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
Bioresour Technol. 2014 Apr;157:14-21. doi: 10.1016/j.biortech.2014.01.027. Epub 2014 Jan 27.
The interaction of recombinant cellulose-binding domains (CBDs) of endoglucanase Cel9B from Paenibacillus barcinonensis with different cotton cellulose allomorphs (I, II and III) has been investigated, in order to bring new insights regarding the CBD adsorption and desorption processes. The highest CBD adsorption capacity was recorded for cellulose I, confirming the affinity of proteins to the most crystalline substrate. The weakening and splitting of the hydrogen bonds within cellulose structure after CBD adsorption, as well as a decrease of the crystallinity degree were identified by ATR-FTIR spectroscopy and XRD. The CBD's adsorption kinetic was shown to be rendered by properties as, specific surface area and porosity, being confirmed by dynamic vapor sorption measurements. An important influence of temperature (25, 37 and 50°C) and/or pH medium (4, 5.5, 7 and 10) on the CBD desorption capacity was confirmed, being related to the hydrophobic interactions formed between the CBD and the cellulose allomorphs.
研究了来自巴氏芽胞杆菌（Paenibacillus barcinonensis）的内切葡聚糖酶 Cel9B 的重组纤维素结合结构域（CBD）与不同棉花纤维素变体（I、II 和 III）的相互作用，以期深入了解 CBD 的吸附和解吸过程。CBD 对纤维素 I 的吸附能力最高，这证实了蛋白质对最结晶底物的亲和力。ATR-FTIR 光谱和 XRD 分析表明，CBD 吸附后纤维素结构内氢键的减弱和分裂以及结晶度的降低。CBD 的吸附动力学受比表面积和孔隙率等特性的影响，这通过动态蒸汽吸附测量得到了证实。还证实了温度（25、37 和 50°C）和/或 pH 介质（4、5.5、7 和 10）对 CBD 解吸能力的重要影响，这与 CBD 与纤维素变体之间形成的疏水相互作用有关。
