College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Bioresour Technol. 2010 Jun;101(11):4062-7. doi: 10.1016/j.biortech.2009.12.145. Epub 2010 Feb 1.
Microbial populations and their relationship to bioconversion during lignocellulosic waste composting were studied by quinone profiling. Nine quinones were observed in the initial composting materials, and 15 quinones were found in compost after 50days of composting. The quinone species Q-9(H2), Q-10 and Q-10(H2) which are indicative of certain fungi appeared at the thermophilic stage but disappeared at the cooling stage. Q-10, indicative of certain fungi, and MK-7, characteristic of certain bacteria, were the predominant quinones during the thermophilic stage and were correlated with lignin degradation at the thermophilic stage. The highest lignin degradation ratio (26%) and good cellulose degradation were found at the cooling stage and were correlated with quinones Q-9, MK-7 and long-chain menaquinones attributed to mesophilic fungi, bacteria and actinomycetes, respectively. The present findings will improve the understandings of microbial dynamics and roles in composting, which could provide useful references for development of composting technology.
采用醌分析方法研究了木质纤维素废物堆肥过程中的微生物种群及其与生物转化的关系。在初始堆肥材料中观察到 9 种醌，在堆肥 50 天后的堆肥中发现 15 种醌。在高温阶段出现但在冷却阶段消失的醌类物质 Q-9(H2)、Q-10 和 Q-10(H2)表明某些真菌的存在。在高温阶段，某些真菌指示的 Q-10 和某些细菌特有的 MK-7 是主要的醌类物质，与高温阶段的木质素降解有关。在冷却阶段发现最高的木质素降解率（26%）和良好的纤维素降解，与分别属于中温真菌、细菌和放线菌的醌 Q-9、MK-7 和长链甲萘醌有关。本研究结果将提高对微生物动力学及其在堆肥中作用的认识，为堆肥技术的发展提供有用的参考。
