Charles University in Prague, Faculty of Medicine in Pilsen, Department of Pathophysiology, Lidická 1, 301 66 Plzen, Czech Republic.
Behav Brain Res. 2010 Jun 26;210(1):8-15. doi: 10.1016/j.bbr.2010.01.035. Epub 2010 Feb 1.
Lurcher mutant mice represent a model of olivocerebellar degeneration. Postnatally, a complete loss of Purkinje cells and secondary reduction of granule cells and inferior olive neurons occurs. Cerebellar ataxia is among the symptoms of degeneration of the cerebellum. The aim of the work was to identify gait parameters which are changed in Lurcher mice due to cerebellar ataxia arising from functional cerebellar decortication, and to assess the correlation between gait parameters, walking speed and performance in rotarod test. We used the adult Lurcher mutant and wild type mice of the B6CBA strain. For gait analysis the CatWalk system was used. Motor functions were examined with the rotarod. Data analysis revealed significant differences between Lurchers and controls in many gait parameters. However, almost all parameters correlated with the walking speed and the differences disappeared after the correction to the walking speed. The question is what is the primary change in Lurchers-whether the walking speed or individual gait parameters. In the rotarod test, the Lurcher mutants revealed significantly worse results than the wild type animals. No correlation between the gait parameters and performance in the rotarod test was found.
Lurcher 突变小鼠是橄榄小脑变性的模型。出生后，浦肯野细胞完全丧失，颗粒细胞和下橄榄神经元减少。小脑共济失调是小脑退化的症状之一。本工作的目的是确定由于功能性大脑皮层切除术引起的小脑共济失调而在 Lurcher 小鼠中改变的步态参数，并评估步态参数、行走速度和旋转棒测试性能之间的相关性。我们使用成年 Lurcher 突变体和野生型 B6CBA 品系小鼠。使用 CatWalk 系统进行步态分析。用旋转棒测试检查运动功能。数据分析显示，Lurcher 与对照组在许多步态参数上存在显著差异。然而，几乎所有参数与行走速度相关，并且在对行走速度进行校正后，差异消失了。问题是 Lurcher 中的主要变化是什么——是行走速度还是个体步态参数。在旋转棒测试中，Lurcher 突变体的表现明显逊于野生型动物。未发现步态参数与旋转棒测试性能之间存在相关性。
