Department of Biochemistry, Sri Venkateswara University, Tirupati 517502, AP, India.
Food Chem Toxicol. 2010 Apr;48(4):1078-84. doi: 10.1016/j.fct.2010.01.029. Epub 2010 Feb 1.
The present study was taken up to identify potent antihyperglycemic fraction from the aqueous extract of Syzygium alternifolium (SA) seeds, using bioassay guided fractionation. The isolated fraction C at a dose of 50 mg/kg.b.w produced the maximum fall of 83% in the blood glucose level in the diabetic rats after 6 h of the treatment. The administration of fraction C (50 mg/kg.b.w) once daily for 30 days in STZ diabetic rats resulted in a significant decrease in blood glucose, glycosylated haemoglobin with a significant rise in plasma insulin level. Further fraction C showed antihyperlipidemic activity as evidenced by significant decrease in serum TC, TG, LDL-C, VLDL-C levels coupled together with elevation of HDL-C level in diabetic rats. A significant decrease in the activities of SGOT, SGPT, ALP and decreased levels of serum urea and creatinine in diabetic treated rats when compared to diabetic untreated rats, indicate the protective role against liver and kidney damage and non-toxic property of the fraction C. A comparison was made between the action of fraction C and antidiabetic drug glibenclamide (20 mg/kg.b.w). The effect of fraction C was more prominent when compared to that of glibenclamide.
本研究旨在从 Syzygium alternifolium（SA）种子的水提取物中鉴定出具有降糖作用的有效部位，采用生物活性导向的分离方法。分离得到的部位 C 在 50mg/kg.b.w 的剂量下，在治疗后 6 小时可使糖尿病大鼠的血糖水平最大降低 83%。在 STZ 糖尿病大鼠中，每日给予部位 C（50mg/kg.b.w）一次，连续 30 天，可显著降低血糖、糖化血红蛋白，并显著升高血浆胰岛素水平。此外，部位 C 还表现出抗高血脂活性，可显著降低糖尿病大鼠血清 TC、TG、LDL-C、VLDL-C 水平，并同时升高 HDL-C 水平。与未治疗的糖尿病大鼠相比，糖尿病治疗大鼠的血清 SGOT、SGPT、ALP 活性降低，血清尿素和肌酐水平降低，表明部位 C 具有保护肝脏和肾脏免受损伤和无毒性的作用。还比较了部位 C 和抗糖尿病药物格列本脲（20mg/kg.b.w）的作用。与格列本脲相比，部位 C 的作用更为显著。
